Elecsys ferritin

Manufactured by Roche

Sourced in Switzerland

The Elecsys® Ferritin is a laboratory equipment product designed for the quantitative determination of ferritin in human serum and plasma. Ferritin is a protein that stores iron and is a useful indicator of the body's iron status.

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Ferritin (2 citations)

7 protocols using elecsys ferritin

COVID-19 Laboratory Parameter Analysis

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The following data of patients were collected: clinical details including comorbidities, COVID severity, and laboratory parameters. White blood cell count (WBC) and platelet count data were obtained from EDTA (BD Vacutainer plastic tubes of 3 mL [buffered K2 EDTA 5.4 mg]) anticoagulated blood samples run on a hematology analyzer (XN‐9000; Sysmex Corp., Kobe, Japan). Coagulation tests were performed on STA R Max3 (Diagnostica Stago, Gennevilliers, France) using citrate vials (BD Vacutainer plastic citrate tubes of 2.7 mL [0.109 M, 3.2% buffered sodium citrate]) and included PT (STA NeoPTimal), international normalized ratio (INR), aPTT (STA Cephascreen), fibrinogen (STA Liquid Fib), and D‐dimer (STA Liatest D‐Dimer). Data of serum creatinine (CREJ2; Roche Diagnostics, Indianapolis, IN, USA), serum urea (Ureal‐Roche Diagnostics), total bilirubin (BILT3, Roche Diagnostics), serum albumin (ALB2, Roche Diagnostics), serum aspartate aminotransferase (AST, Roche Diagnostics), serum alanine aminotransferase (ALT, Roche Diagnostics), serum alkaline phosphatase (ALP2, Roche Diagnostics), C‐reactive protein (Tina‐quant C‐Reactive Protein IV, Roche Diagnostics) and serum ferritin (Elecsys Ferritin, Roche Diagnostics) were obtained from serum samples collected in BD Vacutainer SST tubes and run on a Cobas c701 automated chemistry analyzer (Roche Diagnostics).

Sehgal T., Aggarwal M., Baitha U., Gupta G., Prakash B., Gupta A., Kumar G V., Biswas A., Khan M, & S.h.a.l.i.m.a.r. (2022). Thromboelastography determined dynamics of blood coagulation and its correlation with complications and outcomes in patients with coronavirus disease 2019. Research and Practice in Thrombosis and Haemostasis, 6(1), e12645.

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Comprehensive Laboratory Profiling of COVID-19

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Blood sampling was performed on the day of admission in hospitalized patients and throughout the clinical course in patients with severe disease. Total protein (TP), albumin, AST, lactate dehydrogenase (LDH), creatinine, and C-reactive protein (CRP) were measured by an Aqua-auto Kainos TP-II test, Aqua-auto Kainos ALB (Kainos, Tokyo, Japan), LaboFit AST, CicaFit LD-IFCC (Kanto Chemical Co., Inc., Tokyo, Japan), Signasu-auto CRE (Shino-test, Tokyo, Japan), and CRP-Latex X2 (Denka, Niigata, Japan), respectively, using a LABOSPECT006 (Hitachi High-Tech Co., Tokyo, Japan). PCT and ferritin were measured by Elecsys^® BRAHMS PCT and Elecsys^® Ferritin (Roche Diagnostics K.K., Tokyo, Japan), respectively, using a Cobas 8000 e602 (Roche Diagnostics K.K., Tokyo, Japan). The WBC count, hemoglobin (Hb), total neutrophil count, and total lymphocyte count were measured using a fully automatic blood cell counter XN-3000 (Sysmex Co., Kobe, Japan). HbA1c was measured using an Adams Hybrid AH 8280 (Arkray Factory, Shiga, Japan). The prothrombin time (PT), activated partial thromboplastin time (APTT), and D-dimer level were measured by a Thromborel S, Thrombocheck APTT-SLA, and LIASAUTO D-dimer Neo (Sysmex Co., Kobe, Japan), respectively, using an automatic coagulation analyzer CS-5100 (Sysmex Co., Kobe, Japan).

Yamamoto A., Wada H., Ichikawa Y., Mizuno H., Tomida M., Masuda J., Makino K., Kodama S., Yoshida M., Fukui S., Moritani I., Inoue H., Shiraki K, & Shimpo H. (2021). Evaluation of Biomarkers of Severity in Patients with COVID-19 Infection. Journal of Clinical Medicine, 10(17), 3775.

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Comprehensive COVID-19 Biomarker Monitoring

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Inflammatory and coagulation markers including lactate dehydrogenase (LDH) (LDHI2, cobas® analyzer, Roche Diagnostics), C-reactive protein (CRP) (Tina-quant® C-Reactive Protein IV, cobas® analyzer, Roche Diagnostics), ferritin (Elecsys® Ferritin, cobas® analyzer, Roche Diagnostics), D-dimer (HemosIL® D-dimer HS, ACL TOP 500 analyzer, Instrumentation Laboratory), prothrombin time (PT)/international normalized ratio (INR) (HemosIL® RecombiPlasTin 2G, ACL TOP 500 analyzer, Instrumentation Laboratory), activated partial thromboplastin time (aPTT) (HemosIL® SynthASil, ACL TOP 500 analyzer, Instrumentation Laboratory), and fibrinogen (HemosIL® Fibrinogen-C, ACL TOP 500 analyzer, Instrumentation Laboratory) were measured in real-time by a central clinical laboratory (PPD® Laboratory Services Global Central Labs) at days 0, 7, 14, 21, and 28 and weeks 12 and 24, per the manufacturers’ protocols.

Chew K.W., Moser C., Daar E.S., Wohl D.A., Li J.Z., Coombs R.W., Ritz J., Giganti M., Javan A.C., Li Y., Choudhary M.C., Deo R., Malvestutto C., Klekotka P., Price K., Nirula A., Fischer W., Bala V., Ribeiro R.M., Perelson A.S., Fletcher C.V., Eron J.J., Currier J.S., Hughes M.D, & Smith D.M. (2022). Antiviral and clinical activity of bamlanivimab in a randomized trial of non-hospitalized adults with COVID-19. Nature Communications, 13, 4931.

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Comprehensive Hemostasis Panel Evaluation

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The aPTT (Dade Actin FSL; Siemens, Marburg, Germany), PT (Dade Innovin; Siemens), fibrinogen level (Clauss method, Dade Thrombin Reagent; Siemens), FVIII activity (Dade Actine FS and FVIII deficient plasma; Siemens), D-dimer (INNOVANCE D-dimer; Siemens), antithrombin (INNOVANCE; Siemens), and anti-Xa (Biophen Heparin LRT; Hyphen Biomed, Neuville-Sur-Oise, France) were measured on a Sysmex CS2100i (Sysmex Corporation, Kobe, Hyogo, Japan) hemostasis analyzer. Samples for the anti-Xa test were first diluted 2x with pooled reference plasma containing ∼100% ATIII and the anti-Xa activity was subsequently determined using specific calibration lines for UFH (aXa-UFH) (Biophen UFH Calibrator; Hyphen Biomed) or low-molecular-weight heparin (LMWH) (aXa-LMWH) (Biophen Heparine Calibrator; Hyphen Biomed). The aPTT (Cephascreen; Stago, Paris, France) was also performed on a STA-R Max2 analyzer (Stago). Thrombocyte count was determined using a Sysmex XN-9000 analyzer (Sysmex). C-reactive protein (CRP, third generation, Roche Diagnostics, Basel, Switzerland) and ferritin (Elecsys ferritin, Roche) were performed on the COBAS8000 by Roche Diagnostics.

Streng A.S., Delnoij T.S., Mulder M.M., Sels J.W., Wetzels R.J., Verhezen P.W., Olie R.H., Kooman J.P., van Kuijk S.M., Brandts L., ten Cate H., Lorusso R., van der Horst I.C., van Bussel B.C, & Henskens Y.M. (2020). Monitoring of Unfractionated Heparin in Severe COVID-19: An Observational Study of Patients on CRRT and ECMO. TH Open: Companion Journal to Thrombosis and Haemostasis, 4(4), e365-e375.

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Biomarker Analysis of Blood Samples

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Hemoglobin, ferritin, and CRP concentrations were determined by Medlab Central Medical Testing Laboratory (Palmerston North, New Zealand) using standard procedures. Hemoglobin concentration was determined in EDTA-treated blood using the sodium lauryl sulfate method on an automated Sysmex XN20 analyzer. Ferritin concentration was determined in serum samples using an electrochemiluminescence immunoassay (Elecsys^® Ferritin, Roche Diagnostics International Ltd) on a Roche Cobas e602 analyzer. CRP concentration was determined in serum samples using the immunoturbidometric method (Roche Diagnostics International Ltd) on a Cobas C analyzer.

Henare S.J., Nur Singh N., Ellis A.M., Moughan P.J., Thompson A.K, & Walczyk T. (2019). Iron bioavailability of a casein-based iron fortificant compared with that of ferrous sulfate in whole milk: a randomized trial with a crossover design in adult women. The American Journal of Clinical Nutrition, 110(6), 1362-1369.

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Cardiac and Hepatic Iron Assessment

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Heart and liver iron concentration were measured byT2*MRI (MRI 1.5 Tesla, Achieva, A-series, Philips, Netherland). Results of cardiac T2* were categorized as severe (T2* < 10 ms), moderate (10 < T2* < 14 ms), mild (14 < T2* < 20 ms), and normal (T2* > 20 ms) The results of liver T2* were defined as normal (T2* > 30 ms), mild (T2* > 6.2 ms), moderate (3.1 < T2* < 6.2 ms), severe (2.1 < T2* < 3.1 ms), and very severe (T2* < 2.1 ms) [22 (link)]. Serum ferritin was determined by a electrochemiluminescence immunoassay (Elecsys Ferritin, Roche Diagnostics International Ltd., Switzerland).

Zarghamian P., Azarkeivan A., Arabkhazaeli A., Mardani A, & Shahabi M. (2020). Hepcidin gene polymorphisms and iron overload in β-thalassemia major patients refractory to iron chelating therapy. BMC Medical Genetics, 21, 75.

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Daily Blood Biomarker Monitoring

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Venous blood was drawn daily between 4.30–5.30 a.m. and collected using 2.7 mL BD 3.2% citrate and 5.0 mL BD serum Vacutainer® vacuum tubes. Concentrations of fibrinogen and D-dimer were measured within 2 h of blood collection in citrated plasma, using a Sysmex CS2100i haemostasis analyser (Sysmex Corporation, Kobe, Hyogo, Japan). Detectable fibrinogen concentration had a maximum of 9 g/L. Concentrations of CRP (CRP, third generation, Roche Diagnostics, Basel, Switzerland) and ferritin (Elecsys ferritin, Roche) were measured on the COBAS®8000 by Roche Diagnostics in serum.

Mulder M.M., Brandts L., Brüggemann R.A., Koelmann M., Streng A.S., Olie R.H., Gietema H.A., Spronk H.M., van der Horst I.C., Sels J.W., Wildberger J.E., van Kuijk S.M., Schnabel R.M., ten Cate H., Henskens Y.M, & van Bussel B.C. (2021). Serial markers of coagulation and inflammation and the occurrence of clinical pulmonary thromboembolism in mechanically ventilated patients with SARS-CoV-2 infection; the prospective Maastricht intensive care COVID cohort. Thrombosis Journal, 19, 35.

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