Recombinant human fgf

Manufactured by Thermo Fisher Scientific

Sourced in Germany

Recombinant human FGF is a laboratory reagent that serves as a source of the fibroblast growth factor (FGF) protein, which is involved in various cellular processes. It is produced using recombinant DNA technology.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using recombinant human fgf

Glioblastoma Stem Cell Culture and Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87 cell line was purchased from ATCC and maintained in DMEM (Life Technologies), supplemented with 10% FBS (Sigma-Aldrich), and 1% antibiotic-antimycotic (Life Technologies). The 4 primary glioblastoma stem cell lines (OSU-2, −11, −20, and −53) were isolated from glioblastoma patient tissues and authenticated by a neuropathologist at The Ohio State University. GSCs were maintained in DMEM:F12 (Life Technologies) supplemented with B27 supplement (Gibco), recombinant human FGF (Gibco), recombinant human EGF (Gibco), 10% FBS and 1% antibiotic-antimycotic (Life Technologies). Normal human astrocytes were obtained from Lonza and maintained in Clonetics AGM Astrocyte Growth Medium (Lonza) supplemented with 10% FBS and 1% antibiotic-antimycotic (Life Technologies). All cells were cultured at 37°C under a gas phase of 95% air and 5% CO₂. For calyculin A treatment, cells were treated with 100 nM calyculin A (Santa Cruz) for 30 minutes, and then harvested for western blot. For combination calyculin A and CX-4945 treatment, cells were treated with 10 μM CX-4945 (Selleckchem) for 24 hours, followed by 100 nM calyculin A for 30 minutes.

Bassett E.A., Palanichamy K., Pearson M., McElroy J.P., Haque S.J., Bell E.H, & Chakravarti A. (2018). Calpastatin phosphorylation regulates radiation-induced calpain activity in glioblastoma. Oncotarget, 9(18), 14597-14607.

+ Open protocol

+ Expand

Sphere Formation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 2×10³ cells were seeded into 24-well Corning™ Costar™ Ultra-Low Attachment Microplates and were cultured in serum-free DMEM-F12 (Gibco) supplemented with 1× B27 (Gibco), 20 ng/ml recombinant human EGF (Gibco) and 10 ng/ml recombinant human FGF (Gibco) for 14 days. Images of spheres were taken with a microscope (Olympus). Spheroid numbers and average diameters of six random fields were counted.

Ruan S., Zhang H., Tian X., Zhang Z., Huang H., Shi C., Liu W., Jiang X., Huang D, & Tao F. (2020). PHD Finger Protein 19 Enhances the Resistance of Ovarian Cancer Cells to Compound Fuling Granule by Protecting Cell Growth, Invasion, Migration, and Stemness. Frontiers in Pharmacology, 11, 150.

+ Open protocol

+ Expand

Isolation of Primary Human Neuroblastoma and Kidney Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary human neuroblastoma cells were isolated from fresh tumor tissue from a stage IV N-Myc amplified adrenal mass. Primary human kidney cells were isolated from normal kidney tissue. Minced tissue was dissociated with papain (Worthington Biochemical Corporation, Lakewood, NJ) for 10–15 min at 37°C, triturated using a 5-ml pipette, and re-suspended in complete media (DMEM + 10% FBS). The suspension was filtered twice through 70 μm and 40 μm filters. The suspension was collected in a 15-ml tube and subjected to centrifugation at 1200 rpm for 10 min. The supernatant was aspirated and discarded, and cells were washed with PBS supplemented with Ca²⁺ and Mg²⁺ and subjected to centrifugation at 1000 rpm for 10 min. The supernatant was aspirated, and the cells were re-suspended in DMEM-high glucose with 10% FBS, 100 units/ml penicillin, 100 μg/ml streptomycin, 20 ng/ml EGF (Sigma-Aldrich, St. Louis, MO), 20 ng/ml recombinant human FGF (Preprotech, Rocky Hill, NJ), and fungizone 2.5 μg/ml (UCSF, San Francisco, CA), and seeded in a 10-cm dish for further propagation and characterization. Human tumor samples were obtained with informed consent in accordance with an approved Institutional Review Board Protocol.

Zhao P., Aguilar A.E., Lee J.Y., Paul L.A., Suh J.H., Puri L., Zhang M., Beckstead J., Witkowski A., Ryan R.O, & Saba J.D. (2018). Sphingadienes show therapeutic efficacy in neuroblastoma in vitro and in vivo by targeting the AKT signaling pathway. Investigational new drugs, 36(5), 743-754.

+ Open protocol

+ Expand

HCMV Infection of HFF-1 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HFF-1 cells (ATCC-SCRC-1041, ATCC) were cultivated in Dulbecco’s Modified Eagles Medium Glutamax (Thermo Fisher Scientific), supplemented with 5% FBS superior (Merck) and 5 μl of 10⁶ units/ml recombinant human FGF (PeproTech) / 500 ml Medium. HCMV-pp150-EGFP-gM-mCherry was a kind gift by Christian Sinzger [61 (link)]. The HCMV-TB40-BAC4 was a kind gift by Wolfram Brune [23 (link)]. HCMV-Merlin-pAL1502-WT and HCMV-Merlin-pAL1502-pp150-EGFP-gM-mCherry were a kind gift by Christian Sinzger [74 (link)]. For infection experiments, we used cell-free, infectious supernatant derived from HFFF-TetR cells that was a kind gift of Christian Sinzger. In these cells, RL13 and UL128 expression is suppressed as described by Murrell et al. 2017 [73 (link)] such that virions used for infection were lacking RL13 and UL128. Imaging was done on normal HFF-1 without the tet-repressor such that the analyzed cells expressed RL13 and UL128 [24 (link),73 (link)].
Different multiplicities of infection (MOIs) were used for the infection experiments. In general, low MOI infections were used to avoid artifacts generated by high virus doses. Therefore, whenever possible, we used MOIs between 0.5 and 1. However, for particular experiments, such as bulk assays or electron microscopy, we used MOIs of up to 5. The used MOI is indicated for each experiment.

Flomm F.J., Soh T.K., Schneider C., Wedemann L., Britt H.M., Thalassinos K., Pfitzner S., Reimer R., Grünewald K, & Bosse J.B. (2022). Intermittent bulk release of human cytomegalovirus. PLoS Pathogens, 18(8), e1010575.

+ Open protocol

+ Expand

Multilineage Differentiation of ASCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

For adipogenic, osteogenic, and chondrogenic differentiation, adipose-derived stromal cells (ASCs) (P3, n =3 independent donors) were seeded onto 5 cm cell culture dishes and cultivated in complete α-MEM medium with the addition of recombinant human FGF (50 ng/mL, PeproTech, Hamburg, Germany) until they reached the confluence of 80–90%. Subsequently, the regular culture medium was replaced with respective differentiation media for an additional 2–4 weeks. For adipogenic differentiation, ASCs were cultivated in MesenCultTM adipogenic differentiation medium (StemCell, Cologne, Germany), according to the supplier instructions. For osteogenic differentiation, the cells were cultured with low glucose DMEM (Thermo Fisher Scientific, Basel, Switzerland) supplemented with 0.1 μM dexamethasone, 10 mM glycerol phosphate, 50 μM L-ascorbic acid, 10% FBS, and 50 μg/mL gentamycin (all from Thermo Fisher Scientific, Basel, Switzerland). The chondrogenic differentiation medium consisted of high glucose DMEM (Thermo Fisher Scientific, Basel, Switzerland) supplemented with 50 μg/mL L-ascorbic acid, 40 μg/mL L-proline, 1% ITS™+ Premix (5 μg/mL insulin, 5 μg/mL transferrin, and 5 ng/mL selenious acid), 10 ng/mL TGF-β3, and 50 μg/mL gentamycin.

Ademi H., Michalak-Micka K., Moehrlen U., Biedermann T, & Klar A.S. (2023). Effects of an Adipose Mesenchymal Stem Cell-Derived Conditioned medium and TGF-β1 on Human Keratinocytes In Vitro. International Journal of Molecular Sciences, 24(19), 14726.

+ Open protocol

+ Expand

Cardiac Stem Cell Isolation and Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cardiac derived stem like cells (CTSCs) are isolated from the hearts of C57BL/6 Mice (Jackson) as described previously [2 ]. Briefly, hearts were minced in small pieces and digested in collagenase at 150U mg/ml (Worthington Bio Corp, Lakewood, NJ) for 2 h at 37 °C. After myocyte removal at low-speed centrifugation, the cells are then passed through 100 μm and 50 μm filters (BD biosciences CA), centrifuged at 1200 rpm for 5 min, with resuspension of the pellet in CTSC media consisting of DMEM-F12 (Gibco), 10% Embryonic Stem Cell Fetal Bovine Serum (Gibco), 1% Penicillin-Streptomycin-Glutamine (PSG) (Gibco), 1x Insulin-Transferrin-Selenium (Gibco), Recombinant Human-EGF 10 ng/mL (Peprotech), Recombinant Human-FGF 10 ng/mL (Peprotech), and Leukemia Inhibitory Factor (Millipore Sigma), and incubated in a 37 °C 5% CO₂ incubator. Supernatant was replated 24hrs later in a dish to remove fast adhering cells. CTSCs are infected with Lv-PGK1-LIN28a (CTSC-LIN) and green fluorescent protein (GFP) lentivirus (CTSC-GFP) to create stable cell lines kept between passages 15–22 to minimize variation. Additional details provided in the online data supplement.

Yuko A.E., Carvalho Rigaud V.O., Kurian J., Lee J.H., Kasatkin N., Behanan M., Wang T., Luchesse A.M., Mohsin S., Koch W.J., Wang H, & Khan M. (2021). LIN28a induced metabolic and redox regulation promotes cardiac cell survival in the heart after ischemic injury. Redox Biology, 47, 102162.

+ Open protocol

+ Expand

Isolation and Culture of Cardiac Tissue-Specific Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All CTSCs were isolated from the hearts of C57BL/6 Mice (Jackson). Hearts were subjected to novel tissue digestion method, and separation of various cellular populations by plating, and replating of supernatant to remove fast adhering cells. CTSC Growth Media composition was similar to previously published¹⁹ and included DMEM‐F12 (Gibco), 10% Embryonic Stem Cell Fetal Bovine Serum (Gibco), 1% Penicillin‐Streptomycin‐Glutamine (PSG) (Gibco), 1x Insulin‐Transferrin‐Selenium (Gibco), Recombinant Human‐EGF 10 ng/mL (Peprotech), Recombinant Human‐FGF 10 ng/mL (Peprotech), and Leukemia Inhibitory Factor (Millipore Sigma), and incubated in a 37°C 5% CO₂ incubator. Routine cell culturing passaging included dissociation of cells using 0.25% Trypsin EDTA (Gibco) and centrifugation for 5 minutes at 1500 RPM. Pelleted cells were resuspended in CTSC Growth media and counted using a hemocytometer and plated. For all subsequent experiments, all three CTSCs types were kept between passages 10 and 15.

Kurian J., Yuko A.E., Kasatkin N., Rigaud V.O., Busch K., Harlamova D., Wagner M., Recchia F.A., Wang H., Mohsin S., Houser S.R, & Khan M. (2020). Uncoupling protein 2‐mediated metabolic adaptations define cardiac cell function in the heart during transition from young to old age. Stem Cells Translational Medicine, 10(1), 144-156.

+ Open protocol

+ Expand

Isolation and Expansion of Thymus-Derived Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thymus tissue was collected in sterile PBS from the surgical theater. For comparison studies, the same thymus was cut in two and GMP conditions were compared directly to standard conditions. It was then cleaned and minced in GMP quality Dulbecco's PBS (GMP PBS; Sigma-Aldrich) and enzymatically digested for a 2-h incubation with collagenase NB6 GMP (0.3 mg/mL; Serva) at 37°C. The tissue was passed through a 70-mm cell strainer, which was washed with fresh GMP PBS. Cell suspension was centrifuged at 1500 rpm at 22°C for 5 min. The supernatant was discarded and cells were resuspended in CTS StemPro MSC SFM DMEM (GMP DMEM; Life Technologies) with 10% Human Platelet Lysate (hPl; MACO Biotech) and recombinant human FGF (2.5 ng/mL; Peprotech), and were plated on uncoated plastic. The resulting passage (P0) cultures were kept at 37°C in a humidified atmosphere with 5% CO 2 . After 72-96 h, nonadherent cells were removed with two washes with GMP PBS. Adherent cells were cultured in GMP DMEM, 10% hPl, and FGF with medium changes occurring every 48-72 h and then split when confluence was reached. When confluence was reached, cells were removed from plastic by washing once with GMP PBS, followed by incubation with Trypsin-EDTA (0.05%; Roche), and were then plated at *1 • 10 4 cells/cm 2 in new flasks for the next passage (P1).

Iacobazzi D., Swim M.M., Albertario A., Caputo M, & Ghorbel M.T. (2018). Thymus-Derived Mesenchymal Stem Cells for Tissue Engineering Clinical-Grade Cardiovascular Grafts. Tissue engineering. Part A, 24(9-10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!