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Bms6002

Manufactured by Thermo Fisher Scientific
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The BMS6002 is a laboratory product manufactured by Thermo Fisher Scientific. It is a compact and self-contained unit designed for basic analytical tasks in a laboratory setting. The core function of the BMS6002 is to provide reliable and consistent measurements, but a detailed description of its intended use is not available.

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Lab products found in correlation

Bms603 2, by Thermo Fisher Scientific (5 mentions) Bms607 3, by Thermo Fisher Scientific (3 mentions) Bms618 3, by Thermo Fisher Scientific (3 mentions) Mouse cytokine array panel a, by R&D Systems (2 mentions) Proteome profiler arrays mouse angiogenesis array, by R&D Systems (2 mentions) Cxcl11, by Abcam (2 mentions) Ifn β elisa, by Thermo Fisher Scientific (2 mentions) Cxcl10, by R&D Systems (2 mentions) Kmc0061, by Thermo Fisher Scientific (2 mentions) Rab0477, by Merck Group (1 mentions) Rab0308, by Merck Group (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Kmc4021, by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Ab102531, by Abcam (1 mentions) Coq10, by MyBioSource (1 mentions) Ab118970, by Abcam (1 mentions) Ab102523, by Abcam (1 mentions)

18 protocols using bms6002

1

Measuring Inflammatory Cytokines in Plasma

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To measure the plasma levels of TNFα, IL-1β and IL-6 blood samples were collected at experiment endpoint. ~200 μl of blood was collected and centrifuged at 5,000 rpm for 10 min at 4°C. The plasma levels of TNFα (RAB0477; Sigma-Aldrich, St. Louis, MO, USA), IL-1β (BMS6002; Thermo Fisher Scientific) and IL-6 (RAB0308; Sigma-Aldrich) were determined via ELISA using the manufacturer’s instructions.
Smuder A.J., Roberts B.M., Wiggs M.P., Kwon O.S., Yoo J.K., Christou D.D., Fuller D.D., Szeto H.H, & Judge A.R. (2020). Pharmacological targeting of mitochondrial function and reactive oxygen species production prevents colon 26 cancer-induced cardiorespiratory muscle weakness. Oncotarget, 11(38), 3502-3514.
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2

Embryo Protein Profiling Arrays

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Embryo homogenates prepared as described above (Western blot and antibodies) were analysed with Proteome Profiler Arrays (Mouse Angiogenesis Array, ARY015, and Mouse Cytokine array Panel A, ARY006 both R&D). ELISA kits used were the CXCL4 (R&D, DY595), CXCL11 (Abcam, ab204519), CXCL10 (R&D, DY466-05), IFN Lambda 2/3 (Pbl assay science, 62830-1), IL-1β (ThermoFisher, BMS6002) and IFN-β ELISA (ThermoFisher, 424001).
Peltzer N., Darding M., Montinaro A., Draber P., Draberova H., Kupka S., Rieser E., Fisher A., Hutchinson C., Taraborrelli L., Hartwig T., Lafont E., Haas T.L., Shimizu Y., Böiers C., Sarr A., Rickard J., Alvarez-Diaz S., Ashworth M.T., Beal A., Enver T., Bertin J., Kaiser W., Strasser A., Silke J., Bouillet P, & Walczak H. (2018). LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature, 557(7703), 112-117.
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3

Cytokine ELISA Assay Validation

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Cytokine enzyme-linked immunosorbent assay (ELISA) assays were performed and validated according to manufacturer's instructions, including IL-1β (BMS6002; Thermo Fisher Scientific), IL-6 (KMC0061; Thermo Fisher Scientific), IFNγ (KMC4021; Thermo Fisher Scientific) and TNFα (BMS607-3; Thermo Fisher Scientific).
Heithoff D.M., Pimienta G., Mahan S.P., Yang W.H., Le D.T., House J.K., Marth J.D., Smith J.W, & Mahan M.J. (2022). Coagulation factor protein abundance in the pre-septic state predicts coagulopathic activities that arise during late-stage murine sepsis. EBioMedicine, 78, 103965.
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4

Quantification of Mouse Inflammatory Markers

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Concentration of mouse IL-1β (Thermo Scientific, BMS6002), CCL2 (Thermo Scientific, BMS6005), TNFα (BD Biosciences, 88-7324-22) in 200 μl serum were determined using specific ELISA kits, in accordance with the manufacturer's instructions.
Yang N., Tang Q., Qin W., Li Z., Wang D., Zhang W., Cao X., Lu Y., Ge X., Sun H, & Shen P. (2019). Treatment of obesity-related inflammation with a novel synthetic pentacyclic oleanane triterpenoids via modulation of macrophage polarization. EBioMedicine, 45, 473-486.
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5

Comprehensive Biochemical Analysis of Samples

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The concentrations or activity of amylase (ab102523; Abcam), MPO (EMMPO; Thermo Fisher Scientific), trypsin (ab102531; Abcam), 8-hydroxy-2-deoxyguanosine (K4160; BioVision, Milpitas, CA), HMGB1 (326054329; Sino-Test Corporation, Tokyo, Japan), LDH (ab102526; Abcam), cleaved-caspase3 (DYC835-2; R&D Systems, Minneapolis, MN), p-MLKL (PEL-MLKL-S345-1; RayBiotech Life, Norcross, GA), MDA (ab118970; Abcam), iron (MAK025; Sigma-Aldrich), GSH (CS0260; Sigma-Aldrich), CoQ10 (MBS7233016; MyBioSource, San Diego, CA), tumor necrosis factor (BMS607-3; Thermo Fisher Scientific), IL6 (BMS603-2; Thermo Fisher Scientific), and IL1B (BMS6002; Thermo Fisher Scientific) in the indicated samples were measured using enzyme-linked immunosorbent assay kits according to the manufacturer's guidelines. Data were normalized to protein or DNA concentration.
Liu K., Liu J., Zou B., Li C., Zeh H.J., Kang R., Kroemer G., Huang J, & Tang D. (2021). Trypsin-Mediated Sensitization to Ferroptosis Increases the Severity of Pancreatitis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 13(2), 483-500.
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6

Embryo Protein Profiling Arrays

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Embryo homogenates prepared as described above (Western blot and antibodies) were analysed with Proteome Profiler Arrays (Mouse Angiogenesis Array, ARY015, and Mouse Cytokine array Panel A, ARY006 both R&D). ELISA kits used were the CXCL4 (R&D, DY595), CXCL11 (Abcam, ab204519), CXCL10 (R&D, DY466-05), IFN Lambda 2/3 (Pbl assay science, 62830-1), IL-1β (ThermoFisher, BMS6002) and IFN-β ELISA (ThermoFisher, 424001).
Peltzer N., Darding M., Montinaro A., Draber P., Draberova H., Kupka S., Rieser E., Fisher A., Hutchinson C., Taraborrelli L., Hartwig T., Lafont E., Haas T.L., Shimizu Y., Böiers C., Sarr A., Rickard J., Alvarez-Diaz S., Ashworth M.T., Beal A., Enver T., Bertin J., Kaiser W., Strasser A., Silke J., Bouillet P, & Walczak H. (2018). LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature, 557(7703), 112-117.
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7

Inflammatory Cytokine Profiling in Freeze Injury

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The levels of serum proinflammatory cytokines in uridine or vehicle-treated freeze injured mice were tested by ELISA (Thermo scientific, BMS6002 for IL-1β, KMC0061 for IL-6, EMIL7 for IL-7, BMS6005 for MCP-1 and BMS607-3 for TNF-α). The experiment was performed according to the manufacturers’ instructions. The results were quantified by the microplate reader (Thermo scientific, MK3).
Liu Z., Li W., Geng L., Sun L., Wang Q., Yu Y., Yan P., Liang C., Ren J., Song M., Zhao Q., Lei J., Cai Y., Li J., Yan K., Wu Z., Chu Q., Li J., Wang S., Li C., Han J.D., Hernandez-Benitez R., Shyh-Chang N., Belmonte J.C., Zhang W., Qu J, & Liu G.H. (2022). Cross-species metabolomic analysis identifies uridine as a potent regeneration promoting factor. Cell Discovery, 8, 6.
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8

Multiplex Biomarker Profiling in Mice

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Pooled plasma samples from mice were assayed for leptin (MOB00B, R&D systems), IL-6 (BMS603-2, Invitrogen) IL-1β (BMS6002, Invitrogen), IL-18 (BMS618-3, Invitrogen), and ANGPTL4 (EHANGPTL4, Invitrogen) using ELISA kits.
Cho D.I., Ahn M.J., Cho H.H., Cho M., Jun J.H., Kang B.G., Lim S.Y., Yoo S.J., Kim M.R., Kim H.S., Lee S.J., Dat L.T., Lee C., Kim Y.S, & Ahn Y. (2023). ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation. Experimental & Molecular Medicine, 55(2), 426-442.
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9

Cytokine Profiling Post-Stroke

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The levels of interleukin (IL)-1β, IL-6, and interferon-γ (IFN-γ) derived from the serum and the ipsilesional brain hemisphere (n = 6/group, 48 h after MCAO) were separated using a mouse enzyme-linked immunosorbent assay (ELISA) kit (BMS6002, BMS603-2, and BMS606INST, Invitrogen) according to the manufacturer’s instructions and detected by an ELISA reader at 450 nm. The concentrations of IL-1β, IL-6, and IFN-γ were calculated and displayed as pg/μg total protein.
Chen W.C., Chang L.H., Huang S.S., Huang Y.J., Chih C.L., Kuo H.C., Lee Y.H, & Lee I.H. (2019). Aryl hydrocarbon receptor modulates stroke-induced astrogliosis and neurogenesis in the adult mouse brain. Journal of Neuroinflammation, 16, 187.
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10

Plasma Inflammatory Biomarkers in Mice

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The protein levels of adiponectin (MRP300, R&D systems), interleukin-6 (IL-6, BMS603-2, Invitrogen), IL-1β (BMS6002, Invitrogen), IL-18 (BMS618-3, Invitrogen), and Retnla (MBS9394009, MyBioSource) in mouse plasma were evaluated by using enzyme-linked immunosorbent assay (ELISA) kits. Inflammatory mediators in mouse plasma were evaluated by Multi-Analyte ELISArray kit (Qiagen, 336161).
Kim Y.S., Cho H.H., Cho D.I., Jeong H.Y., Lim S.Y., Jun J.H., Kim M.R., Kang B.G., Cho M., Kang H.J., Kang W.S., Oh G.T, & Ahn Y. (2021). The adipokine Retnla deficiency increases responsiveness to cardiac repair through adiponectin-rich bone marrow cells. Cell Death & Disease, 12(4), 307.
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