The dissolution was performed using Apparatus type 1 (basket method, Varian 705 DS BP Dissolution, Varian, UK). One tablet was placed in 500 mL of water at 37 °C and a rotating speed of 50 rpm. A volume of 4 mL was withdrawn at predetermined time intervals. The concentration of CHD was determined spectrophotometrically at λ254nm (Biochrom WPA Biowave II UV-Spectrophotometry, UK). The test was performed in triplicate.
Wpa biowave 2 uv spectrophotometry
Manufactured by Harvard Bioscience
Sourced in United Kingdom
The WPA Biowave II UV-Spectrophotometry is a laboratory instrument designed for spectrophotometric analysis. It measures the absorption of ultraviolet and visible light by samples, which can be used to determine the concentration of specific substances within the sample.
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Lab products found in correlation
3 protocols using wpa biowave 2 uv spectrophotometry
1
Dissolution of Chlorhexidine Digluconate
2
Solubility of Chlorhexidine Dihydrochloride
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The solubility of CHD was investigated in ultra-pure water and a phosphate buffer saline (PBS) solution pH 6.8, as a simulated salivary pH to select the most appropriate dissolution medium with a sink condition.
The solubility of CHD was determined by adding excess CHD to 3 mL of water or a PBS solution at pH 6.8. The samples were shaken at 37 ± 0.1 °C for 24 h. The suspensions were filtered through a 0.45 µm Millipore membrane filter and the concentration of CHD was measured by UV detection at λ254 nm (Biochrom WPA Biowave II UV-Spectrophotometry, UK).
The solubility of CHD was determined by adding excess CHD to 3 mL of water or a PBS solution at pH 6.8. The samples were shaken at 37 ± 0.1 °C for 24 h. The suspensions were filtered through a 0.45 µm Millipore membrane filter and the concentration of CHD was measured by UV detection at λ254 nm (Biochrom WPA Biowave II UV-Spectrophotometry, UK).
3
Dissolution Profiling of Tablet Formulation
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The dissolution was performed using Varian 705 DS BP Dissolution Apparatus type I (basket method) (Varian, UK). One tablet of each formulation was placed in 500 mL of ultrapure distilled water at 37 ± 0.1 °C, with the basket rotating at 50 ± 1 rpm. At predetermined time intervals, 4 mL was withdrawn from the dissolution medium and replaced with the same volume of fresh media. The CHD concentration was determined spectrophotometrically at λ254 (Biochrom WPA Biowave II UV-Spectrophotometry, UK).
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