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Axioplan 2 immunofluorescent microscope

Manufactured by Zeiss
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The Axioplan II is an immunofluorescent microscope designed for use in research laboratories. It features a high-precision optical system and advanced illumination technology to facilitate the visualization and analysis of fluorescently labeled samples.

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Lab products found in correlation

Transwell insert, by BD (5 mentions) Transwell chamber, by BD (2 mentions)

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Axioplan 2 microscope (979 citations) Axioplan 2 (859 citations) Axioplan microscope (451 citations) Axioplan (304 citations) Immunofluorescent microscope (5 citations)

5 protocols using axioplan 2 immunofluorescent microscope

1

Transwell Migration and Invasion Assay

Cited 1 time
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Transwell migration and invasion assays were performed as described in Okimoto et al.35 (link) Briefly, 8-μm-pore Matrigel coated (invasion) or non-coated (migration) Transwell inserts (BD Biosciences) were added at the top of a Transwell chamber filled with 10% FBS, RPMI media. To each insert, 2.4 × 104 cells in serum-free media were added. The Transwell chambers were incubated for 20 hrs at 37 °C in the incubator. Cells that did not migrate through the pore or invade the matrigel were scraped off; the membranes were fixed in methanol for 15 min and then stained with crystal violet for 30 min. The surface of the membrane was imaged in 5 distinct fields, with a Zeiss Axioplan II immunofluorescent microscope at 10×. Invasion and migration were assessed counting the average imaged cells in the 5 regions. Results presented are from three independent experiments.
Blakely C.M., Watkins T.B., Wu W., Gini B., Chabon J.J., McCoach C.E., McGranahan N., Wilson G.A., Birkbak N.J., Olivas V.R., Rotow J., Maynard A., Wang V., Gubens M.A., Banks K.C., Lanman R.B., Caulin A.F., John J.S., Cordero A.R., Giannikopoulos P., Simmons A.D., Mack P.C., Gandara D.R., Husain H., Doebele R.C., Riess J.W., Diehn M., Swanton C, & Bivona T.G. (2017). Evolution and clinical impact of co-occurring genetic alterations in advanced-stage EGFR-mutant lung cancers. Nature genetics, 49(12), 1693-1704.
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2

Transwell Invasion and Migration Assay

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RPMI with 10% FBS was added to the bottom well of a trans-well chamber. 2.5×104 cells resuspended in serum free media was then added to the top 8 µm pore matrigel coated (invasion) or non-coated (migration) trans-well insert (BD Biosciences). After 20 hours, non-invading cells on the apical side of inserts were scraped off and the trans-well membrane was fixed in methanol for 15 minutes and stained with Crystal Violet for 30 minutes. The basolateral surface of the membrane was visualized with a Zeiss Axioplan II immunofluorescent microscope at 10×. Each trans-well insert was imaged in five distinct regions at 10× and performed in triplicate. % invasion was calculated by dividing the mean # of cells invading through Matrigel membrane / mean # of cells migrating through control insert.
Okimoto R.A., Breitenbuecher F., Olivas V.R., Wu W., Gini B., Hofree M., Asthana S., Hrustanovic G., Flanagan J., Tulpule A., Blakely C.M., Haringsma H.J., Simmons A.D., Gowen K., Suh J., Miller V.A., Ali S., Schuler M, & Bivona T.G. (2016). Inactivation of Capicua drives cancer metastasis. Nature genetics, 49(1), 87-96.
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3

Transwell Invasion and Migration Assay

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RPMI with 10% FBS was added to the bottom well of a trans-well chamber. 2.5×104 cells resuspended in serum free media was then added to the top 8 µm pore matrigel coated (invasion) or non-coated (migration) trans-well insert (BD Biosciences). After 20 hours, non-invading cells on the apical side of inserts were scraped off and the trans-well membrane was fixed in methanol for 15 minutes and stained with Crystal Violet for 30 minutes. The basolateral surface of the membrane was visualized with a Zeiss Axioplan II immunofluorescent microscope at 10×. Each trans-well insert was imaged in five distinct regions at 10× and performed in triplicate. % invasion was calculated by dividing the mean # of cells invading through Matrigel membrane / mean # of cells migrating through control insert.
Okimoto R.A., Breitenbuecher F., Olivas V.R., Wu W., Gini B., Hofree M., Asthana S., Hrustanovic G., Flanagan J., Tulpule A., Blakely C.M., Haringsma H.J., Simmons A.D., Gowen K., Suh J., Miller V.A., Ali S., Schuler M, & Bivona T.G. (2016). Inactivation of Capicua drives cancer metastasis. Nature genetics, 49(1), 87-96.
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4

Invasion and Migration Assay

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RPMI with 10% FBS was added to the bottom well of a trans-well chamber. 2.5×10^4 cells resuspended in serum free media was then added to the top 8 µm pore matrigel coated (invasion) or non-coated (migration) trans-well insert (BD Biosciences). After 20 hr, non-invading cells on the apical side of inserts were scraped off and the trans-well membrane was fixed in methanol for 15 min and stained with Crystal Violet for 30 min. The basolateral surface of the membrane was visualized with a Zeiss Axioplan II immunofluorescent microscope at 5×. Each trans-well insert was imaged in five distinct regions at 5×and performed in triplicate. % invasion was calculated by dividing the mean # of cells invading through Matrigel membrane / mean # of cells migrating through control insert.
Gupta N., Song H., Wu W., Ponce R.K., Lin Y.K., Kim J.W., Small E.J., Feng F.Y., Huang F.W, & Okimoto R.A. (2022). The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression. eLife, 11, e77072.
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5

Transwell Migration and Invasion Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Transwell migration and invasion assays were performed as described in Okimoto et al.35 (link) Briefly, 8-μm-pore Matrigel coated (invasion) or non-coated (migration) Transwell inserts (BD Biosciences) were added at the top of a Transwell chamber filled with 10% FBS, RPMI media. To each insert, 2.4 × 104 cells in serum-free media were added. The Transwell chambers were incubated for 20 hrs at 37 °C in the incubator. Cells that did not migrate through the pore or invade the matrigel were scraped off; the membranes were fixed in methanol for 15 min and then stained with crystal violet for 30 min. The surface of the membrane was imaged in 5 distinct fields, with a Zeiss Axioplan II immunofluorescent microscope at 10×. Invasion and migration were assessed counting the average imaged cells in the 5 regions. Results presented are from three independent experiments.
Blakely C.M., Watkins T.B., Wu W., Gini B., Chabon J.J., McCoach C.E., McGranahan N., Wilson G.A., Birkbak N.J., Olivas V.R., Rotow J., Maynard A., Wang V., Gubens M.A., Banks K.C., Lanman R.B., Caulin A.F., John J.S., Cordero A.R., Giannikopoulos P., Simmons A.D., Mack P.C., Gandara D.R., Husain H., Doebele R.C., Riess J.W., Diehn M., Swanton C, & Bivona T.G. (2017). Evolution and clinical impact of co-occurring genetic alterations in advanced-stage EGFR-mutant lung cancers. Nature genetics, 49(12), 1693-1704.
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