Rosa yfp

OF1 and C57/B6J2 mice were obtained from Charles River Breeding Laboratories. Prdm1MEGFP transgenic mice were kindly provided by Mitinori Saitou; Lef1tm1 Rug mice were provided by Rudolf Grosschedl and Werner Held. Sox2Cre, Sox2CreERT2, Prdm1Cre, Wnt1Cre, Prdm1 CA, and ROSAYFP mice were obtained from the Jackson Laboratory (Bar Harbor, United States) (Table 1). The strains carrying the Cre recombinase and ROSAYFP were kept in heterozygosity, while the conditional knockout was performed in homozygosity. Genotyping was conducted using the primers listed in Table 2. All animals were maintained in a 12 h light cycle providing food and water ad libitum. Mice were sacrificed by intra-peritoneal (i.p.) injection of pentobarbital. Experiments were conducted in accordance with the EU Directive (86/609/EEC) for the care and use of laboratory animals and that of the Swiss Confederation. Mating of adult female and male mice was carried out overnight. Time-pregnant mice were sacrificed by injection of pentobarbital and uteri with embryos were removed by dissection.

Lgr5-EGFP-IRES-CreER^T2 (Barker et al. 2007 (link)), Lgr6-EGFP-IRES-CreER^T2 (Snippert et al. 2010a (link)), Rosa-YFP (Srinivas et al. 2001 (link)), and Rosa-tdTomato (Madisen et al. 2010 (link)) mice were obtained from the Jackson Laboratory. K14CreER^T2 (Li et al. 2000 (link)) mice were provided by P. Chambon; Rosa-Confetti (Snippert et al. 2010b (link)) mice were provided by H. Clevers, K14rtTA transgenic mice (Nguyen et al. 2006 (link)) were provided by Elaine Fuchs, TetO-Cre mice (Perl et al. 2002 (link)) were provided by Andreas Nagy, and Sox9CreER^T2 mice (Kopp et al. 2011 (link)) were provided by Frédéric Lemaigre. The generation of K5CreER^T2 (Van Keymeulen et al. 2011 (link)), K19CreER^T (Means et al. 2008 (link)), and K8rtTA (Watson et al. 2015 (link)) mice was described previously. All experimental mice used for experiments on breasts were female, and all experimental mice used for prostate experiments were male. All animals were mixed strains. No statistical methods were used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment. Mice colonies were maintained in a certified animal facility in accordance with European guidelines. The experiments were approved by the local ethical committee (Comission d'Ethique et du Bien-Etre Animal [CEBEA]).

C57BL/6ScNj, beta-actinGFP (GFP), Prx1^Cre, Pax7^CreERT2, Rosa-tdTomato-EGFP (Rosa^mTmG) and Rosa^YFP mice were obtained from Jackson Laboratory (Bar Harbor, ME) and maintained on a C57BL6/J background. Mice were bred and kept under controlled pathogen conditions in separated ventilated cages with controlled humidity an ambient temperature, with 12:12-hour light:dark cycles and free access to food and water in the animal facilities of IMRB, Creteil and Imagine Institute, Paris. All experiments were performed in compliance with procedures approved by the Paris Est Creteil and Paris University Ethical Committees. Animals used for all experiments were males or females 10–14-week-old and experimental groups were homogeneous in terms of animal gender and age. No specific randomization methods were used. Sample labeling allowed blind analyses.

Prx1-Cre (stock 005584), Rosa-CreER (stock 008463), Rosa-YFP (stock 006148), and Rosa-tdTomato (stock 007914) mice were obtained from The Jackson Laboratory. Bap1^fl/fl mice were generated using the gene trap embryonic stem cell line (HEPD0526_2_G01) made by EUCCOM (the International Knockout Mouse Consortium). In the mutant embryonic stem (ES) cell line, the gene trap cassette was integrated in the fifth intron of the murine Bap1 locus (Supplemental Figure 1A). The FRT cassette was removed by crossing with flippase-transgenic mouse. Cre-mediated deletion of exons in Prx1-CreBap1^fl/fl mice resulted in a frameshift mutation, leading to premature termination by open reading frame. Genotyping primers were designed outside the nondeleted common exon (P1) and were used in combination with primers (P2) within and outside the deleted exon (P3), which could represent genomic deletion (Supplemental Figure 1, A and C). All mouse lines were backcrossed into C57BL/6J background and were housed under specific pathogen–free conditions and handled according to the guidelines of the Institutional Animal Care and Use Committee (IACUC) at Seoul National University. Experiments were carried out with male and female mice, except for the weight analyses and whole transcriptomic analysis, where only male mice were used. No sex-specific differences were observed.