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2 protocols using vitamin d3

1

Vitamin D Compound Cell Culture Study

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Almost all compounds were purchased from Selleck (Shanghai, China), including vitamin D3 (S4063), vitamin D2 (S4035), calcitriol (S1466), calcifediol (S1469), calcipotriene (S3739), doxercalciferol (S1467), lithocholic acid (S4003), lovastatin (S2061) and cholesterol (S4154). Tacalcitol (HY‐32337) was purchased from MedChemExpress (Shanghai, China). These compounds were added to the cell culture medium at the concentrations indicated, as appropriate. If not labeled, the concentrations of vitamin D3, calcifediol and calcitriol used for BNI274 were 5 μm, and for BNI423 were 2.5 μm.
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2

Cell Line Authentication and Reagent Use

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Cell lines derived from Prof. Shao and Prof. Tong lab were used. All cell lines were authenticated and cultured according to ATCC standards. Reagents used in cell culture were as follows: Fetal bovine serum (SH30406.05, HyClone), penicillin/streptomycin (15140148, Thermo), erastin (S7242, Selleck), ferrostatin-1 (S7243, Selleck), vitamin D3 (S4063, Selleck), mitoxantrone (S1210, Selleck), MG132 (S2619, Selleck), puromycin (S7417, Selleck), cycloheximide (S7418, Selleck), bafilomycin A1 (S1413, Selleck), ketoconazole (HY-B0105, MCE), and CoCl2 (232696, Sigma-Aldrich).
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