Astra software v 6

Manufactured by Wyatt Technology

Sourced in United States

Astra software v. 6.1 is a data analysis and visualization software used for characterizing macromolecules and nanoparticles. The software provides tools for processing and interpreting data from light scattering, sedimentation, and other analytical techniques.

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Lab products found in correlation

Optilab t rex refractometer, by Wyatt Technology (1 mentions) Minidawn treos light scattering detector, by Wyatt Technology (1 mentions) Superdex 200 increase 10 300 gl column, by GE Healthcare (1 mentions) Lc 10advp, by Shimadzu (1 mentions) Dawn dsp laser light scattering photometer, by Wyatt Technology (1 mentions)

Close spelling variants of this product

ASTRA 6 software (413 citations) ASTRA software (357 citations) ASTRA VI software (30 citations) ASTRA v. 6 software (23 citations)

5 protocols using astra software v 6

Characterization of DUSP26-N (C152S) Protein

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After system equilibration in 50 mM Bis-Tris (pH 6.0), 1 mM DTT at RT, the samples were analyzed with three detectors in series, namely, the UV and light-scattering detectors of the DAWM HELEOS II system (Wyatt Technology) coupled to a refractive-index detector (Optilab T-rEX refractometer; all from Wyatt Technology Corp., Wyatt Technology Europe GmbH, In der Steubach, Germany). As previously described [42 (link)–44 (link)], analysis was performed at RT by injecting the DUSP26-N (C152S) protein sample of 100 μL (2.5 mg/mL) into the SEC-MALS system (WTC-015S5 column, Wyatt Technology) at Korea Basic Science Institute in a mobile phase consisting of 50 mM Bis-Tris (pH 6.0) and 1 mM DTT at a flow rate of 0.5 mL/min. Data were analyzed and weight-averaged molar masses were calculated using ASTRA software (V6, Wyatt Technology).

Won E.Y., Lee S.O., Lee D.H., Lee D., Bae K.H., Lee S.C., Kim S.J, & Chi S.W. (2016). Structural Insight into the Critical Role of the N-Terminal Region in the Catalytic Activity of Dual-Specificity Phosphatase 26. PLoS ONE, 11(9), e0162115.

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SEC-MALS Analysis of Full-Length McsB Protein

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SEC-MALS analysis of 25-μl full-length McsB samples at ~100 μM (~4 mg/ml) was performed using a Superdex200 increase 5/150 GL column installed on an ÄKTA ETTAN LC system (GE Healthcare Life Sciences) and equilibrated in 50 mM Tris, pH 7.5, 50 mM NaCl. The flow rate of 0.33 ml/min was used. The column was connected to a miniDAWN TREOS light scattering detector (Wyatt Technologies). Average molecular mass across the central parts of main peaks was calculated using ASTRA software v6 (Wyatt Technologies).

Suskiewicz M.J., Hajdusits B., Beveridge R., Heuck A., Vu L.D., Kurzbauer R., Hauer K., Thoeny V., Rumpel K., Mechtler K., Meinhart A, & Clausen T. (2019). Structure of McsB, a protein kinase for regulated arginine phosphorylation. Nature chemical biology, 15(5), 510-518.

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Molecular Weight Determination of NADK2

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The measurements were done using Superdex 200 Increase 10/300 GL column (GE Healthcare) with a flow-rate of 0.5 mL/min, connected to Agilent^™ Infinity II HPLC system coupled to miniDAWN TREOS (Waytt Technology) multi-angle light scattering detector and Optilab T-rEX (Waytt Technology) refractometer. A 20 μL sample of NADK2 at 3.5 mg/mL was injected and eluted in 50 mM Tris pH 7.8, 150 mM NaCl. The data were analyzed using the ASTRA software v. 6.1 (Wyatt Technology). The calculation of protein molecular weight was obtained from light scattering. The refractive index increment used was dn/dc 0.185 mL/g.

Grider A., Bailey L.B, & Cousins R.J. (1990). Erythrocyte metallothionein as an index of zinc status in humans.. Proceedings of the National Academy of Sciences of the United States of America, 87(4), 1259-1262.

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Molecular Weight Determination by SEC-MALS

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Size exclusion chromatography with multi angle light scattering (SEC-MALS) was used to measure the molecular weight, as previously described [28 (link)]. The system consisted of a mobile phase reservoir, an on-line degasser, an HPLC isocratic pump (LC-10ADVP, Shimadzu, Kyoto, Japan), an autoinjector (SCL-10AV, Shimadzu), a pre-column and two serially connected columns: TSK 6000 PWXL and TSK 5000 PWXL (Toso Haas, Stuttgard, Germany). The columns were followed by two serially connected detectors; a Dawn DSP laser light scattering photometer (Wyatt Technology, Santa Barbara, CA, USA) (λ₀ = 0.664 nm) and an Optilab DSP differential refractometer (Wyatt Technology, Santa Barbara, CA, USA). 0.15 M NaNO₃ with 0.01 M NaEDTA, pH 6 was used as the mobile phase. 20% acetonitrile was added to the mobile phase for the alginate grafted with β-CyD. All samples were dissolved in the mobile phase (0.25–7.5 mg/mL) and filtered (pore size 0.8 µm) prior to injection. The injection volume was adjusted to obtain an optimal light scattering signal and to avoid overloading. Astra software v. 6.1 (Wyatt Technology, Santa Barbara, CA, USA) was used to collect and process the obtained data, using a refractive index increment (dn/dcµ) of 0.150 mL/g for alginate samples [28 (link)].

Dalheim M.Ø., Omtvedt L.A., Bjørge I.M., Akbarzadeh A., Mano J.F., Aachmann F.L, & Strand B.L. (2019). Mechanical Properties of Ca-Saturated Hydrogels with Functionalized Alginate. Gels, 5(2), 23.

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SEC-MALS Analysis of MeOTyr Samples

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The molecular weight of MeOTyr coupled samples was analyzed by Size Exclusion Chromatography with Multi Angle Light Scattering (SEC-MALS). Samples and standards dissolved in the mobile phase (0.05M Na 2 SO 4 with 0.01M EDTA, pH 6.0) were injected on an HPLC system consisting of a mobile phase reservoir, an on-line degasser, an HPLA isocratic pump, an autoinjector, a precolumn and two serially connected columns (TSK 5000 PWXL, and TSK 4000 PWXL). The column outlet was connected to two serially connected detectors, a Dawn DSP multiangle laser light scattering photometer (Wyatt, USA) (λ 0 = 0.633 nm) followed by an Optilab DSP differential refractometer (P-ml/min. Injection volume and sample concentration was adjusted to obtain an optimal light scattering signal without influencing the RI profile (overloading). All samples were filtered (pore size 0.8 µm)
prior to injection. Astra software v. 6.1 (Wyatt, USA) was used to collect and process the data obtained from the light scattering and the differential refractometer, using a refractive index increment (at constant chemical potential), (dn/dc) µ of 0.150 ml/g [28] for alginate samples.

Dalheim M.Ø., Vanacker J., Najmi M.A., Aachmann F.L., Strand B.L, & Christensen B.E. (2016). Efficient functionalization of alginate biomaterials. Biomaterials, 80.

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