The food-grade enzyme Alcalase 2.4 L was provided by Novozymes (Bagsvaerd, Denmark). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), pyrocatechol violet, ferrozine, angiotensin-converting enzyme (ACE) and hippuryl-histidyl-leucine (HHL) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cu(NO3)2, Mg(NO3)2, Mn(NO3)2, Fe(NO3)3, NaNO3 KNO3, Ni(NO3)2 and Cr(NO3)3 Pb(NO3)2 and Cd(NO3)2 solutions were purchased from Merck (Darmstadt, Germany). All other chemicals were of analytical grade.
Fe no3 3
Manufactured by Merck Group
Sourced in United States, Germany
Fe(NO3)3, also known as ferric nitrate, is an inorganic chemical compound. It is a crystalline solid with a reddish-brown appearance. Fe(NO3)3 is commonly used in various laboratory applications, serving as a reactant or precursor for other chemical processes.
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Lab products found in correlation
Cu no3 2, by Merck Group (8 mentions)
Pb no3 2, by Merck Group (6 mentions)
Soluble starch, by Merck Group (6 mentions)
Thiamine pyrophosphate, by Merck Group (6 mentions)
D glucose, by Carl Roth (6 mentions)
L glutamine, by Carl Roth (6 mentions)
4 aminobenzoic acid, by Merck Group (6 mentions)
Vitamin b12, by Merck Group (6 mentions)
Sodium chloride (nacl), by Merck Group (5 mentions)
Zn no3 2, by Merck Group (5 mentions)
Sodium hydroxide (naoh), by Merck Group (5 mentions)
K2hpo4, by Carl Roth (5 mentions)
Kh2po4, by Carl Roth (5 mentions)
Sodium chloride (nacl), by Carl Roth (5 mentions)
Thiamine hcl, by Carl Roth (5 mentions)
Proteose peptone no 3, by BD (5 mentions)
Mn no3 2, by Merck Group (4 mentions)
Cd no3 2, by Merck Group (4 mentions)
Ni no3 2, by Merck Group (4 mentions)
Agno3, by Merck Group (4 mentions)
27 protocols using fe no3 3
1
Antioxidant and ACE Inhibition Assays
2
Synthesis and Characterization of Metal Nanoparticles
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Chloroauric acid trihydrate (HAuCl4∙3H2O), Silver nitrate (AgNO3), L-ascorbic acid (AA), Sodium hydroxide (NaOH), anion salts (Na2SO4, NaCl, NaNO3, Na2HPO4, NaHS∙H2O, NaF, NaBr, NaI and KSCN), cation salts (Al(SO4)2∙18H2O, CuSO4∙5H2O, ZnSO4∙7H2O, HgCl2, Pb(NO3)2, Fe(NO3)3, FeSO4, CaCO3, MgSO4∙7H2O, NiSO4∙6H2O, CrN3O9∙9H2O, CdSO4, CoSO4∙7H2O) were obtained from Merck, Germany. All chemicals used in experiment were analytical reagent (AR grade).
3
Synthesis of Silver-Decorated Hematite Nanospheres
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Silver decorated hematite nanospheres (α-Fe2O3/Ag) were synthesized following a modified chemical co-precipitation method [38 (link)], as described in our previous work [39 (link)]. Briefly, a 125 mL solution of 0.1 mmol L−1 of Fe(NO3)3 (Merck Co.) and AgNO3 (Merck Co.) ranging from 0 up to 7.4 mmol L−1 was added dropwise (0.5 mL min−1) into 250 mL of 0.3 mol L−1 Na2CO3 (Riedel- deHaen Co.) solution, in the presence of 0.5 g of polyethylene glycol 2000 (Merck Co.) as surfactant. The pH of the solution was maintained constant at 10.8 during the co-precipitation process by the dropwise (1.5 mL min−1) addition of 0.1 mol L−1 Na2CO3. A brownish precipitate was formed immediately upon dropwise addition of the metal precursor. The mixture was kept under continuous magnetic stirring at 80 °C for 60 min, and the precipitate aged for an additional 12 h in static conditions. The solid was separated by centrifugation, washed three times with ethanol, and dried at 80 °C for 4 h. The recovered solid was milled using an agate mortar and uniformed using stainless steel test sieve, ø 100 μm. Then, the samples were calcined at 400 °C to ensure the formation of hematite as a pure metal oxide phase decorated with silver.
4
Cinnamaldehyde-Induced Apoptosis in Cancer Cells
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The chemicals for synthesis, FeSO4.7H2O, Fe(NO3)3, NH4OH, glycine and pluronic F-127, were procured from Merck. Dulbecco's Modified Eagle's Medium (DMEM), penicillin/streptomycin and L-glutamine were obtained from Gibco BRL, CA, USA. Cinnamaldehyde, foetal bovine serum (FBS), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylthiazolium bromide (MTT), FCCP and JC-1 were procured from Sigma-Aldrich (St. Louis, MO, USA). Neutral red and potassium ferrocyanide were obtained from Sd Fine-Chem. Ltd. Mumbai, India. Antibodies against caspase-3, VEGF and α-tubulin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). All other common reagents were procured from Qualigens fine chemicals (Mumbai, India).
5
Functionalized Silica and Florisil for Metal Extraction
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Silica and florisil were impregnated with crown ether (dibenzo-18-crown-6) and Fe(III) ions using the dry method. Over 1 g of solid support was added to 0,01 g of crown ether (Merck, Germany) used as solvent 25 mL of acetone (VWR Prolabo Chemicals, France). In these solutions, Fe(III) ions were also added in order to obtain a concentration of 400 mg/L from a standard solution of 1 g/L Fe(NO3)3 (Merck, Germany) in 0,5 mol/L HNO3 solution. The sample was kept in contact for 24 h. After that, it was dried at 323.15 K for 24 h.
6
Synthesis of Cationic Styryl Dyes
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2,3,3-Trimethylindolenin (98%), 1,1,2-trimethylbenz[e]indole (98%), 2-bromoethanol (95%), 9-anthraldehyde, and 4-nitrobenzaldehyde (98%), which were used for the synthesis of (E)-1-(2-hydroxyethyl)-3,3-dimethyl-2-(4-nitrostyryl)-3H-indol-1-ium (OX1-Nitro), (E)-3-(2-hydroxyethyl)-1,1-dimethyl-2-(4-nitrostyryl)-1H-benzo[e]indol-3-ium (OX2-Nitro), (E)-2-(2-(anthracen-9-yl)vinyl)-1-(2-hydroxyethyl)-3,3-dimethyl-3H-indol-1-ium (OX1-Anthracene), and (E)-2-(2-(anthracen-9-yl)vinyl)-3-(2-hydroxyethyl)-1,1-dimethyl-1H-benzo[e]indol-3-ium (OX2-Anthracene) were purchased from Sigma-Aldrich. The metal salts including Zn(NO3)2, Cu(NO3)2, Ni(NO3)2, Co(NO3)2, Pb(NO3)2, Fe(NO3)3, Cd(NO3)2, AgNO3, and Hg(NO3)2 were supplied by the Merck Chemical Company and used for the preparation of aqueous solutions of metal ions. Distilled-deionized (DI) water was used in all the recipes, and all the materials were used without further purifications.
7
Glucose and H2O2 Detection Assay
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D-(+)-Glucose was purchased from Ajex Finechem. Galactose, sucrose, maltose, L-ascorbic acid, and uric acid were obtained from BDH Chemical. H2O2 (30 wt%), standard cation and anions (KCl, CaCl2, NaCl, and Fe(NO3)3) were purchased from Merck. GOx and HRP were purchased from Sigma-Aldrich. Artificial urine was obtained from Carolina Biological Supply Company (Burlington, NC, USA). All chemicals used in this experiment were of analytical reagent (AR) grade and were prepared with deionized water from a Mill-Q purification system (resistance ≥18 MΩ cm -1 ). Lastly, Whatman No. 1 filter paper was purchased from Cole-Parmer (VernonHills, IL).
UV-visible absorption spectra were measured with a UVvisible spectrophotometer (Lambda 35, Perkin15 Elmer Instruments, USA) using quartz cuvettes (1.0 cm path length) to characterize AgNPs. The morphology and size of silver nanoparticles were determined using a Transmission Electron Microscope (TEM, JEOL). Glucose and H2O2 detection were achieved under a black box to protect the decomposition of H2O2. With the completed enzymatic reaction, we recorded the results with a scanner (Scx-3405, Samsung).
UV-visible absorption spectra were measured with a UVvisible spectrophotometer (Lambda 35, Perkin15 Elmer Instruments, USA) using quartz cuvettes (1.0 cm path length) to characterize AgNPs. The morphology and size of silver nanoparticles were determined using a Transmission Electron Microscope (TEM, JEOL). Glucose and H2O2 detection were achieved under a black box to protect the decomposition of H2O2. With the completed enzymatic reaction, we recorded the results with a scanner (Scx-3405, Samsung).
8
Synthesis of Graphene Quantum Dots
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Cu(NO3)2 (99.99%), Fe(NO3)3 (98%), NaOH (98%) solution, acetone (99.5%), and chloroform (99%) were obtained from Merck company and utilized without further purification. For the synthesis of graphene quantum dots, citric acid monohydrate (Merck, 98%) was used. Pirimicarb was ordered from Mahan Co. (Tabriz, Iran). Ethylene glycol dimethacrylate (EGDMA) (97.5%) and Methacrylic acid (MAA) (99.6%) were acquired from Sigma-Aldrich, and Azobisisobutyronitrile (AIBN) (96%) was purchased from Merck.
9
Tetracycline Adsorption on Metal Oxides
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Chemicals and reagents used here were all of analytical grade. The hydrochloride salt of tetracycline (96% pure) was purchased from Sigma-Aldrich Co. Other chemicals, including Fe(NO3)3, ZnCl2, CuCl2, AlCl3, NaCl, HCl and NaOH, were obtained from Merck. All solutions were prepared using 18 MΩ deionized water. All reaction vessels were acid-washed using 1 M HCl and rinsed with deionized water prior to the experiments. All experiments were carried out in a 0.01 M NaCl background, and the pH values were adjusted using 0.01 M HCl or NaOH.
10
Macro and Trace Element Standards
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The macro- (Ca, Mg, Na, and K) and trace (Fe, Cu, Mn, and Zn) element standards (CaCO3, Mg band, NaCl, KCl, Fe(NO3)3, Cu(NO3)2, Mn(NO3)2, and Zn(NO3)2; >99% purity), as well as LaCl2 and CsCl (>99% purity) were purchased from Merck (Darmstadt, Germany). Pepsin (from porcine stomach mucosa; 1.470 units/mg prot; P-7000); pancreatin (from porcine pancreas, activity equivalent to 4 x U.S.P. specifications; P-1750); and porcine bile (B-8631) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). All other reagents were purchased from Panreac Química S.L.U. (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Indianapolis, IN, USA).
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