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Automated cellular image system 3 acis 3

Manufactured by Agilent Technologies
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Sourced in Denmark

The Automated Cellular Image System III (ACIS III) is a lab equipment product designed for automated cellular image analysis. It captures and processes high-resolution images of cells and tissues for various applications. The ACIS III is capable of automated cell detection, classification, and quantification.

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Lab products found in correlation

Pk 6101, by Vector Laboratories (2 mentions) Ab205921, by Abcam (1 mentions) E cadherin, by BD (1 mentions) Mircury lna microrna ish optimization kit ffpe, by Qiagen (1 mentions) U6 probe, by Qiagen (1 mentions)

4 protocols using automated cellular image system 3 acis 3

1

Breast Cancer Tissue Microarray Analysis

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Breast cancer tissue microarrays (n = 129) were collected from Shanghai Jiaotong University, and written informed consent was obtained from patients in all cases at the time of enrollment. Tissues specimens were incubated with antibodies against E-cadherin (BD Biosciences, #610182, 1:100), STT3A (Santa Cruz, #SC-100796, 1:50), STT3B (abcam, #ab122351, 1:150), non-phospho (active) β-catenin (Cell Signaling, #8814, 1:100), or PD-L1 (abcam, #ab205921, 1:100) at 4 °C overnight. The specimens were then treated with biotinylated secondary antibody, followed by incubations with avidin–biotin–peroxidase complex solution for 1 h at room temperature. Visualization was performed using 3-amino-9-ethylcarbazole solution. Counterstaining was carried out using Mayer’s hematoxylin. All immunostained slides were scanned on the Automated Cellular Image System III (ACIS III, Dako, Denmark) for quantification by digital image analysis. The score of protein expression was calculated from both the percentage (0–100%) of immunopositive cells and the immunostaining intensity (0: negative, 1: low, 2: medium, 3: high). The number from percentage × intensity represents an arbitrary quantitative score64 (link). According to the scores, the protein expression correlations between different proteins were analyzed.
Hsu J.M., Xia W., Hsu Y.H., Chan L.C., Yu W.H., Cha J.H., Chen C.T., Liao H.W., Kuo C.W., Khoo K.H., Hsu J.L., Li C.W., Lim S.O., Chang S.S., Chen Y.C., Ren G.X, & Hung M.C. (2018). STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nature Communications, 9, 1908.
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2

Immunohistochemical Analysis of RNASEH2B in Prostate Adenocarcinoma

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The human prostate adenocarcinoma tissue microarray (PR806) were purchased from US Biomax. It contains 80 prostate adenocarcinoma samples and 20 normal prostate tissue samples. Samples were deparaffinized and rehydrated. Antigen retrieval was done using 0.01 M sodium citrate buffer (pH 6.0) in a microwave oven. To block endogenous peroxidase activity, the sections were treated with 1% hydrogen peroxide in methanol for 30 min. After 1 h of preincubation in 10% normal goat serum to prevent nonspecific staining, the samples were incubated with anti-RNASEH2B (1:50; Cat# HPA040084, Sigma), overnight at 4°C. The sections were then incubated with a biotinylated secondary antibody (1:200; Vector Laboratories, PK-6101) and incubated with avidin–biotin peroxidase complex solution (1:100) for 30 min at room temperature. Color was developed with the 3-amino-9-ethylcarbazole (AEC) solution. Counterstaining was carried out using Mayer’s hematoxylin. All immunostained slides were scanned on the Automated Cellular Image System III (ACIS III, Dako) for quantification by digital image analysis. A total score of protein expression was calculated from both the percentage of immune-positive cells and the immunostaining intensity. High and low protein expressions were defined using the mean score of all samples as a cutoff point.
Wang C., Wang G., Feng X., Shepherd P., Zhang J., Tang M., Chen Z., Srivastava M., McLaughlin M.E., Navone N.M., Hart G.T, & Chen J. (2018). Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition. Oncogene, 38(14), 2451-2463.
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3

Quantitative miRNA Expression Analysis

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The double (5′ and 3′) digoxigenin (DIG)-labeled miR-100 probe and U6 probe were purchased from Exiqon. The normal mammary tissue and breast tumor sections were purchased from Origene (normal: CS807851; tumor: CS704488 and CS 711714). The tissue microarray (TMA) slide was purchased from Biomax (BR1006). In situ hybridization was performed according to the protocol of the miRCURY LNA microRNA ISH Optimization Kit (FFPE) (Exiqon). The stained slide was scanned on the Automated Cellular Image System III (ACIS III, Dako, Denmark) for quantification by digital image analysis. The color threshold was set up and standardized for all samples, and the color intensity was automatically scored for all individual cores on the TMA slide. The expression level was calculated from the score of color intensity and normalized to the internal control U6.
Chen D., Sun Y., Yuan Y., Han Z., Zhang P., Zhang J., You M.J., Teruya-Feldstein J., Wang M., Gupta S., Hung M.C., Liang H, & Ma L. (2014). miR-100 Induces Epithelial-Mesenchymal Transition but Suppresses Tumorigenesis, Migration and Invasion. PLoS Genetics, 10(2), e1004177.
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4

Immunohistochemical Analysis of RNASEH2B in Prostate Adenocarcinoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human prostate adenocarcinoma tissue microarray (PR806) were purchased from US Biomax. It contains 80 prostate adenocarcinoma samples and 20 normal prostate tissue samples. Samples were deparaffinized and rehydrated. Antigen retrieval was done using 0.01 M sodium citrate buffer (pH 6.0) in a microwave oven. To block endogenous peroxidase activity, the sections were treated with 1% hydrogen peroxide in methanol for 30 min. After 1 h of preincubation in 10% normal goat serum to prevent nonspecific staining, the samples were incubated with anti-RNASEH2B (1:50; Cat# HPA040084, Sigma), overnight at 4°C. The sections were then incubated with a biotinylated secondary antibody (1:200; Vector Laboratories, PK-6101) and incubated with avidin–biotin peroxidase complex solution (1:100) for 30 min at room temperature. Color was developed with the 3-amino-9-ethylcarbazole (AEC) solution. Counterstaining was carried out using Mayer’s hematoxylin. All immunostained slides were scanned on the Automated Cellular Image System III (ACIS III, Dako) for quantification by digital image analysis. A total score of protein expression was calculated from both the percentage of immune-positive cells and the immunostaining intensity. High and low protein expressions were defined using the mean score of all samples as a cutoff point.
Wang C., Wang G., Feng X., Shepherd P., Zhang J., Tang M., Chen Z., Srivastava M., McLaughlin M.E., Navone N.M., Hart G.T, & Chen J. (2018). Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition. Oncogene, 38(14), 2451-2463.
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