KURAMOCHI cells (JCRB Cell Bank, JCRB0098) were grown in RPMI1640 (Corning, 10–040-CV) with 10% fetal bovine serum (FBS) (Gibco, 10,270,106), 1% penicillin–streptomycin (PS) (Gibco, 15,140,122). CAOV3 cells (ATCC, HTB-75) were grown in DMEM (Corning, 15–013-CV) with 10% FBS, 1% PS, and 1% GlutaMAX (Gibco, 35,050,038).
Kuramochi cells
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in United States
KURAMOCHI cells are a cell line derived from a human gastric cancer. They are maintained in tissue culture and can be used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Caov 3 cells, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Rpmi 1640, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thomas Scientific (1 mentions)
Ovcar 3 cells, by American Type Culture Collection (1 mentions)
Ham s f12 medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Nacalai Tesque (1 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions)
Ovcar3, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Caov3, by American Type Culture Collection (1 mentions)
Cov362, by European Collection of Authenticated Cell Cultures (1 mentions)
Doxycycline, by Topscience (1 mentions)
Carboplatin, by Selleck Chemicals (1 mentions)
A2780, by European Collection of Cell Cultures (1 mentions)
Cov 504, by European Collection of Cell Cultures (1 mentions)
4 protocols using kuramochi cells
1
Cell Culture Protocols: KURAMOCHI and CAOV3
2
Culturing High-Grade Serous Carcinoma Cell Lines
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All reagents were obtained from Life Technologies (Carlsbad, CA) unless otherwise indicated. Murine oviductal cells (MOE) and murine ovarian surface epithelial cells (MOSE) were obtained from Dr. Barbara Vanderhyden at the University of Ottawa. MOE and MOSE cells were cultured as previously described [34 (link)]. OVCAR4 cells were obtained from the National Cancer Institute from the Division of Cancer Treatment and Diagnosis Tumor Repository. Kuramochi cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB). OVCAR4 and Kuramochi cells were cultured using RPMI 1640 media, supplemented with 10% FBS (Denville Scientific, Holliston, MA) and 1% pen/strep. OVCAR3 cells were purchased from the American Type Culture Collection (ATCC). OVCAR3 cells were cultured using MEM, supplemented with 20% FBS (Denville Scientific, Holliston, MA), 0.05 mg/mL Insulin, 1% non-essential amino acids, 1% sodium pyruvate, 1% L-glutamine, and 1% pen/strep. OVCAR3 and Kuramochi cells have been verified by STR analysis. The molecular profiles and in-vivo tumor growth capabilities of all three HGSC lines used in this study have been previously characterized [35 (link)–37 ].
3
Ovarian Cancer Cell Lines and Control Models
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The following six cell lines were used as ovarian cancer models: KURAMOCHI, COV362, CAOV3, OVCAR3, OV90, and SKOV3 lines. OV90, CAOV3, OVCAR3, and SKOV3 cells were purchased from the American Type Culture Collection; COV362 cells were from the European Collection of Authenticated Cell Cultures; and KURAMOCHI cells were from the Japanese Collection of Research Bioresources Cell Bank. Cell lines were maintained by culturing in their optimal medium and conditions according to the suppliers’ recommendations. Four noncancer cell lines, including HOSE1, HOSE2, HFF2T, and MTK, were also used. HOSE1 and HOSE2 are the human ovarian surface epithelia immortalized with mutant CDK4, cyclin D1, and telomerase reverse transcriptase (TERT) (47 (link)). HFF2T is human foreskin fibroblasts immortalized with TERT (48 (link), 49 (link)). MTK cells derived from human greater omental mesothelium are immortalized with mutant CDK4, cyclin D1, and TERT by using lentivirus-mediated gene transfer. HOSE1 and HOSE2 were cultured in a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM) and Ham’s F-12 medium (Thermo Fisher Scientific Inc., MA, USA). HFF2T was cultured in DMEM (4500 mg/liter of glucose) (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS). MTK cells were cultured in RPMI 1640 (Nacalai Tesque) supplemented with 10% FBS and 1% PS.
4
Cell Lines and Reagents for Ovarian Cancer Research
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A2780 and COV504 cells were purchased from the European Collection of Cell Cultures (Salisbury, UK). OVCAR8 cells were obtained from the National Cancer Institute (USA), KURAMOCHI cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, Japan), and DOV13 cells were purchased from BioVector (BioVector NTCC). All other human cell lines were from the American Type Culture Collection. Short Tandem Repeat (STR) authentication of cell lines was done by the authors. Mouse ovarian carcinoma cell line ID8 was purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). The B16-OVA cell line (C57BL/6 mouse melanoma) was constructed by overexpression of OVA in B16 cells, and B3Z hybridoma cells were a gift from Nilabh Shastri (University of California, Berkeley, California, USA) (59 (link)). Cell lines were cultured in DMEM or RPMI 1640 medium supplemented with 10% FBS, 1% penicillin-streptomycin (Gibco), and maintained at 37°C in a humidified 5% CO2 atmosphere and routinely tested negative for mycoplasma. Carboplatin were purchased from Selleck Chemicals. Doxycycline and verteporfin were from TopScience, respectively. Mouse IFN-β was obtained from R&D Systems. Stock solutions were diluted and stored according to the manufacturer’s protocols.
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