1 14c acetic acid

¹⁴C-labeled compounds were used because of their longer half-lives (T_1/2=5,700 years) compared to those of ¹⁸F- (T_1/2=110 min) or ¹¹C-labeled compounds (T_1/2=20 min). Trans-1-amino-3-fluoro-[1-¹⁴C]cyclobutanecarboxylic acid (anti-[¹⁴C]FACBC, 2.09 GBq/mmol) was radiolabeled by Sekisui Medical Co. Ltd. as described previously [10 (link)]. L-[¹⁴C (U)]glutamine ([¹⁴C]Gln, 10.17 GBq/mmol) was purchased from PerkinElmer. L-[methyl-¹⁴C]methionine ([¹⁴C]Met, 2.04 GBq/mmol), [¹⁴C (U)]2-deoxy-2-fluoro-D-glucose ([¹⁴C]FDG, 11.10 GBq/mmol), [methyl-¹⁴C]choline chloride ([¹⁴C]choline, 2.04 GBq/mmol), [1-¹⁴C]acetic acid, and sodium salt ([¹⁴C]acetate, 1.85 GBq/mmol) were purchased from American Radiolabeled Chemicals.
The human prostate cancer cell lines LNCaP-FGC (LNCaP) and DU145 and fetal bovine serum (FBS) were purchased from American Type Culture Collection (ATCC). Cell culture medium and antibiotics were purchased from Life Technologies. 5α-Dihydrotestosterone (DHT) and bicalutamide were purchased from Sigma-Aldrich.

ELT3 cells were labeled with [1-¹⁴C]acetic acid or [1-¹⁴C]FACE (0.5 μCi /ml) obtained from American Radiolabeled Chemicals (ARC, MO) for 6 hr, then washed 3 times and collected for lipid extraction using methanol/chloroform/H₂O (2:1:0.5 v/v/v). Radioactivity was counted on a Packard Tri-Carb Liquid Scintillation Analyzer.