Trypsin ethylenediamine tetraacetate trypsin edta

Dulbecco’s modified Eagle medium (DMEM) was purchased from BioWhittaker (Walkersville, MD, USA). DMEM powder, fetal bovine serum (FBS), and trypsin-ethylenediamine tetraacetate (trypsin-EDTA) were obtained from Gibco BRL (San Francisco, CA, USA). Calcium chloride dehydrate (CaCl₂·2H₂O), sodium bicarbonate, dimethyl sulphoxide (DMSO), and thiazolyl blue tetrazolium bromide (MTT) were bought from Sigma-Aldrich (St. Louis, MO, USA). Human breast cancer cell line MCF-7 (ATCC^® HTTB-22 TM Homo sapiens mammary gland) was used. The AF 488 negative siRNA and all functionally validated siRNAs (Table 1) used in this study against calcium ion channels and transporter genes were from QIAGEN (Valencia, CA, USA). Polyclonal antibodies for human p42/44 Erk, pan-Akt, phospho-Akt, and phospho-p42/44 Erk were purchased from Cell Signaling Technology (CST), Massachusetts. Proteins raised in rabbit were procured from Thermo Scientific.

Dulbecco’s modified Eagle medium (DMEM) was purchased from BioWhittaker (Walkersville, MD, USA). DMEM powder, foetal bovine serum (FBS) and trypsin-ethylenediamine tetraacetate (trypsin-EDTA) were obtained from Gibco BRL (Carlsbad, CA, USA). Calcium chloride dihydrate (CaCl₂·2H₂O), sodium bicarbonate, dimethyl sulphoxide (DMSO) and thiazolyl blue tetrazolium bromide (MTT) were from Sigma-Aldrich (St. Louis, MO, USA). Streptavidin from Streptomyces avidinii, Poly(ethylene glycol) 2-aminoethyl ether biotin, and fibronectin (derived from human plasma) were obtained from Sigma Aldrich (St. Louis, MO, USA). The chemotherapy drugs, gemcitabine and anastrozole, were purchased from Sigma Aldrich (St. Louis, MO, USA).