Grs taq dna polymerase

α-d-Mannopyranosylphenyl isothiocyanate (MPITC) and commercial branched PEI with average Mw 25 kDa and GRS Taq DNA polymerase were purchased from Sigma Aldrich Chemicals (St. Louis, MO, USA). R8 peptide was custom synthesized from GCC Biotech Pvt. Ltd. (West Bengal, India). The TripleXtractor used in RNA extraction was obtained from GRISP (Porto, Portugal). DMEM-F12 and DMEM-HG were purchased from GIBCO (Waltham, MA, USA). Sodium bicarbonate was obtained from MP Biomedicals (Santa Ana, CA, USA). DAPI was obtained from Invitrogen (Carlsbad, CA, USA). FITC was obtained from Alfa Aesar (Lanchashire, UK). Agarose and GreenSafe were obtained from NZYtech (Lisbon, Portugal).

Medical grade high molecular weight (HMW) chitosan, with a molecular weight range between 200 and 500 kDa, was purchased from Heppe Medical (Halle, Germany). Low molecular weight (LMW) chitosan, with a molecular weight range between 50 and 190 kDa, chitosan of 5 kDa molecular weight and GRS Taq DNA polymerase were purchased from Sigma Aldrich Chemicals (St. Louis, MO, USA). chitosan of 20 kDa molecular weight was purchased from Gletham Life Sciences (Corsham, UK). Sodium tripolyphosphate (TPP) was obtained from Across Organics (Geel, Belgium). TripleXtractor used in RNA extraction was obtained from GRISP (Porto, Portugal). DMEM-F12 was purchased from GIBCO (Waltham, MA, USA). MEM-α was purchased from HyClone classical media (Boston, MA, USA). Sodium bicarbonate was obtained from MP Biomedicals (Santa Ana, CA, USA). Agarose and GreenSafe was obtained from NZYtech (Lisbon, Portugal).

Medical grade CS 95/1000 (with a molecular weight range between 200 and 500 kDa was) purchased from Heppe Medical (Berlin, Germany), TPP was obtained from Acros Organics (Geel, Belgium), 35% hydrochloric acid, sodium hydroxide palettes, and glacial acetic acid were all acquired from VWR (Prague, Czech Republic). The following solutions were freshly prepared by using deionized water from VWR (Prague, Czech Republic): 2 M HCl, 10 M NaOH, 1%, and 2% (v/v) acetic acid. GRS Taq DNA polymerase was purchased from Sigma Aldrich Chemicals (St. Louis, MO, USA). TripleXtractor used in RNA extraction was obtained from GRISP (Porto, Portugal). DMEM-F12 and DMEM-HG were purchased from GIBCO (Waltham, MA, USA). Sodium bicarbonate was obtained from MP Biomedicals (Santa Ana, CA, USA). Agarose, MgCl₂ and GreenSafe were obtained from NZYtech (Lisbon, Portugal). All solutions were freshly prepared by using ultra-pure grade water, purified with a Milli-Q system from Millipore (Billerica, MA, USA).