The residual concentration of OG and RhB in the reaction system were detected using UV spectrophotometer, at detection wavelengths 475 nm and 554 nm, respectively. The residual concentrations of ACT, STZ and BPA were determined form high performance liquid chromatography (LC-20A, Shimadzu, Japan) with a photodiode array detector (SPDM20A). A Zorbax SB-C18 column (4.6 × 250 mm, 5 μm) (Agilent, USA) was used at a column temperature of 30 °C. A mixture of methanol (A)/0.3% formic acid solution (B) (30 : 70, v/v, for ACT and STZ; 70 : 30, v/v, for BPA) was used as the mobile phase at a flow rate of 1.0 mL min−1. Total organic carbon (TOC) was determined using a TOC analyzer (Elementar, Germany). The degradation products were detected using a 7890B/5977C GC-MS (Agilent, USA). The reactive oxide species (ROS) generated form the system was detected using an EPR spectrometer (Bruker A320, USA).
7890b 5977c gc ms
Manufactured by Agilent Technologies
Sourced in United States
The 7890B/5977C GC-MS is a gas chromatography-mass spectrometry system manufactured by Agilent Technologies. It is designed to analyze and identify the chemical composition of various samples by separating and detecting individual compounds.
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2 protocols using 7890b 5977c gc ms
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Spectroscopic and Chromatographic Analysis of Organic Pollutants
2
Chemical Profiling of Housefly Puparia
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For identification of the chemicals, the non-polar fraction of the extract obtained from the puparial halves of HwAM, HwAMF (2 µl of each) as well as the extract from 2 individuals each of both adult males and females (separately dipped in 20 µl of Hexane for 10 minutes and concentrated to 2 µl under Nitrogen stream), were all separately injected (split-less mode) into a gas-chromatograph coupled with Mass spectrometer (Agilent 7890B, 5977C GC-MS). The machine had a capillary column, HP-5MS (Agilent J&W), with an operational mode of electron impact ionization at 70eV (Quadrupole temperature of 150°C).
The inlet temperature and the auxiliary line temperature were maintained at 320 o C, and Helium was used as the carrier gas with an avg. velocity = 36.2 cm/sec. The oven temperature was programmed from 40°C with a hold of 5 minutes, increased from 40°C to 300°C at 4 °C/min with a final hold for 25 minutes. Hunter Workstation Software vB.08.00 (Agilent Technologies). For calculating the relative abundance of each identified peak, each was divided by the area of the most abundant peak within each sample (i.e., nC-29 in HwAM, nC-31 in HwAMF, as well as adult female and MeC31 (7-) in adult males). The peak ratios relative to the highest peak (taken as 100 %) were transformed into percentages for subsequent statistical analysis.
The inlet temperature and the auxiliary line temperature were maintained at 320 o C, and Helium was used as the carrier gas with an avg. velocity = 36.2 cm/sec. The oven temperature was programmed from 40°C with a hold of 5 minutes, increased from 40°C to 300°C at 4 °C/min with a final hold for 25 minutes. Hunter Workstation Software vB.08.00 (Agilent Technologies). For calculating the relative abundance of each identified peak, each was divided by the area of the most abundant peak within each sample (i.e., nC-29 in HwAM, nC-31 in HwAMF, as well as adult female and MeC31 (7-) in adult males). The peak ratios relative to the highest peak (taken as 100 %) were transformed into percentages for subsequent statistical analysis.
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