The largest database of trusted experimental protocols

5 protocols using bgj 398

1

Targeting VEGF and FGF Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bevacizumab, an immunoglobulin (Ig) G1 monoclonal antibody targeting VEGF-A, was obtained from Chugai Pharmaceutical (Tokyo, Japan). For in vivo studies, bevacizumab was diluted in phosphate-buffered saline (PBS). BGJ-398, a FGFR-specific inhibitor, was purchased from Chemietek (Indianapolis, IN). For the in vitro studies, 4 mM stock solutions were prepared in DMSO. For the in vivo studies, BGJ-398 was formulated in PEG300/D5W (2:1). FGF2-neutralizing antibody was purchased from R&D systems (Minneapolis, MN). AMD3100, a selective CXCR4 antagonist, was purchased from Sigma (St Louis, MO). For in vitro studies, 10 mg ml−1 stock solutions were prepared in PBS. SU5416, a VEGFR-specific inhibitor, was purchased from Abcam (Cambridge, MA). For the in vivo studies, SU5416 was formulated in DMSO. An anti-mouse IL-2 receptor β-chain monoclonal antibody, TM-β1, was supplied by Drs M. Miyasaka and T. Tanaka (Osaka University, Osaka, Japan).
+ Open protocol
+ Expand
2

Xenograft Model of FGFR Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) mice (obtained from the Jackson Laboratories), were maintained as a breeding colony. All experiments were conducted under Augusta University IACUC approved protocols. Female, 6–8 week old mice were used in all xenograft experiments. Mice were engrafted with 1–2 × 106 cells via tail vein injection. All mice were treated with either drug, or vehicle control (PEG300:acetic buffer = 1:1), orally using a gavage needle once per day. All treatments were performed 5 days per week for 4 weeks.
Ponatinib was provided by Ariad Pharmaceuticals Inc. (Cambridge, MA). PD173074 was purchased from Cayman Chemical, AZD4547 and BGJ398 from ChemieTek, JNJ-42756493 from Active Biochem and TKI258 from LC Laboratories. E3810 was provided by EOS pharmaceuticals. All drugs were dissolved in DMSO and stored at −80°C before use. For drug treatments, cells were seeded at 3,000–10,000 cells/well, depending on the cell line, in 96-well plates and incubated overnight. Cells were then treated with either DMSO (control) or different FGFR inhibitors as indicated in the results section at concentrations defined by the experiments. Cell viability was determined using CellTiter-Glo® luminescence cell viability kits (Promega) and a SpectraMax® M5e (Molecular Probes) luminescence plate reader as described previously.8 (link)
+ Open protocol
+ Expand
3

Preparation of Targeted Kinase Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
AZD4547 and BGJ398 were purchased from the ChemieTek, JNJ42756493 from Active Biochem, TKI258 from LC Laboratories and PD173074 from Cayman Chemical. All drugs were diluted in DMSO, aliquoted and stored as 10 mM stocks at −80°C.
+ Open protocol
+ Expand
4

Modeling Metastatic Lung and Breast Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Parental (that is, scrambled shRNA) and FGFR1-manipulated D2.A1 cells were injected into the lateral tail veins of 5-week-old female BALB/C mice (The Jackson Laboratory, Bar Harbor, ME, USA). Where indicated, mice bearing D2.A1 pulmonary tumors were treated daily with BGJ-398 (ChemieTek, Indianapolis, IN, USA) or PF-573271 (PF271; Pfizer Pharmaceuticals, New York, NY, USA) at 50 mg/kg by oral gavage. Alternatively, Cdh1 reporter 4T1 cells (1 × 104 cells) were engrafted onto the mammary fat pads of 4-week-old BALB/c mice. Circulating 4T1 tumor cells were isolated from the inferior vena cava at the time of necropsy using 3% sodium citrate. Following lysis of red blood cells, circulating tumor cells were selected for with 5 μg/ml Zeocin (the selectable marker for firefly luciferase). Luciferase-expressing NME cells (1 to 2 × 106 cells) were engrafted onto the mammary fat pads of 5-week-old female nu/nu mice. All bioluminescent images were captured using a Xenogen IVIS 200 preclinical imaging system (Caliper Life Sciences/PerkinElmer, Hopkinton, MA, USA) within the Small Animal Imaging Resource Center at the Case Comprehensive Cancer Center as previously described [5 (link),21 (link),23 (link)].
+ Open protocol
+ Expand
5

Inhibitors of Angiogenesis and Fibrosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nintedanib and SB431542 were obtained from Boehringer Ingelheim GmbH & Co. KG (Biberach, Germany). SU5416, a VEGFR-specific inhibitor, was purchased from Abcam (Cambridge, MA). BGJ-398 and imatinib were purchased from Chemietek (Indianapolis, IN). Bleomycin (BLM) was purchased from Nippon Kayaku Co. (Tokyo, Japan).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!