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4 methylum belliferyl β d glucuronide hydrate 4 mu g

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4-methylum-belliferyl-β-D-glucuronide hydrate (4-MU-G) is a fluorogenic substrate used in enzymatic assays. It is a hydrated form of 4-methylumbelliferyl β-D-glucuronide, a compound that emits fluorescence upon enzymatic cleavage.

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Lab products found in correlation

4 methylumbelliferone 4 mu, by Merck Group (2 mentions) Hplc grade methanol, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Niflumic acid, by Merck Group (1 mentions) Propofol, by Merck Group (1 mentions) Fluconazole, by Merck Group (1 mentions) Zidovudine, by Merck Group (1 mentions) Uridine diphosphate glucuronic acid, by Merck Group (1 mentions) Methylumbelliferone, by Merck Group (1 mentions) Propofol glucuronide, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Trifluoperazine dihydrochloride tfp, by Merck Group (1 mentions) Alamethicin, by Merck Group (1 mentions) Qubit protein assay kit, by Thermo Fisher Scientific (1 mentions) Alamethicin, by BD (1 mentions) Morphine, by Merck Group (1 mentions) Triamcinolone, by Merck Group (1 mentions) Ugt1a9, by BD (1 mentions) Ugt1a1, by BD (1 mentions) Ugt1a3, by BD (1 mentions)

2 protocols using 4 methylum belliferyl β d glucuronide hydrate 4 mu g

1

Synthesis and Glucuronidation of NAF Enantiomers

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R(+)-NAF (>99.5% ee) and S(-)-NAF (>99.5% ee) (Figure 1) were synthesized according to previous methods (Shivani et al., 2007 (link)). The internal standard (IS) Z10 (CN201110148322.7) was obtained from our laboratory. HPLC-grade methanol, acetonitrile, and acetic acid were obtained from Merck (Darmstadt, Germany). Uridine diphosphate glucuronic acid (UDPGA), alamethicin, Tris, magnesium chloride, methylum-belliferone (MU), 4-methylumbelliferone (4-MU), 4-methylum-belliferyl-β-D-glucuronide hydrate (4-MU-G), propofol, propofol glucuronide, zidovudine, zidovudine glucuronide, fluconazole, and niflumic acid, trifluoperazine dihydrochloride (TFP), and TFP-glucuronide (TFP-G) were purchased from Sigma–Aldrich (St. Louis, MO, United States). Recombinant human UGTs (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 1A10, 2B4, 2B7, 2B15, and 2B17) and the commercially available microsomes from human liver (HLMs), intestine (HIMs), and kidney (HKMs), as well as those from rat liver (RLMs), intestine (RIMs), and kidney (RKMs), were purchased from BD Gentest (Woburn, MA, United States). Protein contents of the microsomes were used according to the manufacturers’ instructions. Ultrapure water was used in all experiments (Millipore, Billerica, MA, United States).
Liu X.W., Rong Y., Zhang X.F., Huang J.J., Cai Y., Huang B.Y., Zhu L., Wu B., Hou N, & Luo C.F. (2018). Human UDP-Glucuronosyltransferase 2B4 and 2B7 Are Responsible for Naftopidil Glucuronidation in Vitro. Frontiers in Pharmacology, 8, 984.
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2

Glucuronidation Assay for Drug Metabolism

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Aprepitant, aprepitant-13C2,d2, aprepitant-β-glucuronide, 3’-azido-3’-deoxythymidine (AZT), and AZT-3-β-D-glucuronide (AZT-G) were purchased from Toronto Research Chemicals, Inc. (North York, ON, CA). Morphine, Morphine-6-β-D-glucuronide (M-6-G), 10,11-dihydrocarbamazapine, 4-methylumbelliferone (4-MU), triamcinolone, and 4-methylumbelliferyl-β-D-glucuronide hydrate (4-MU-G) were purchased from Sigma-Aldrich (St. Louis, MO). Uridine 5'-diphospho-glucuronic acid (UDPGA) (UGT Rxn mix Solution A), 250 mM Tris-HCl (pH 7.5) buffer mix with 40 mM MgCl2 and 0.125 mg/ml alamethicin (UGT Rxn mix Solution B), UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B4, UGT2B7, UGT2B10, UGT2B15, UGT2B17, UGT control Supersomes, and human intestinal microsomes (HIM) were purchased from BD Biosciences (Woburn, MA). Human liver microsomes (HLM) were processed through Dr. Mary Relling's laboratory at St. Jude Children's Research Hospital (Memphis, TN). Livers were obtained through the Liver Tissue Cell Distribution System (funded by #NO1-DK-9-2310) and the Cooperative Human Tissue Network. Protein concentrations were measured using the Qubit® protein assay kit (Invitrogen Life Technologies, Grand Island, NY).
House L., Ramirez J., Seminerio M., Mirkov S, & Ratain M.J. (2015). IN VITRO GLUCURONIDATION OF APREPITANT: A MODERATE INHIBITOR OF UGT2B7. Xenobiotica; the fate of foreign compounds in biological systems, 45(11), 990-998.
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