Analytical grade chemicals with maximum purity were used in this study. Silver nitrate (AgNO3) and bacterial culture media were purchased from Himedia Laboratories Ltd., India. Uropathogens responsible for UTIs such as S. aureus, K. pneumoniae, E. coli, P. aeruginosa, and E. faecalis, were granted by the K.A.P. Viswanatham Medical College, Tiruchirappalli – 620 001, Tamil Nadu.
Bacterial culture media
Manufactured by HiMedia
Sourced in India
Bacterial culture media are lab equipment used to cultivate and grow various types of bacteria. They provide the necessary nutrients and growth conditions for bacterial colonies to thrive in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Restriction enzyme, by New England Biolabs (2 mentions)
Silver nitrate agno3, by HiMedia (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dna modifying enzymes, by New England Biolabs (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Low melting point agarose, by Merck Group (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Tris buffer, by Merck Group (1 mentions)
Ni nta agarose, by Qiagen (1 mentions)
Molecular biology kits, by Qiagen (1 mentions)
Dntps and enzymes, by New England Biolabs (1 mentions)
Deoxynucleotide triphosphates dntps, by New England Biolabs (1 mentions)
5 protocols using bacterial culture media
1
Uropathogens Antimicrobial Screening
2
Molecular Biology Reagents and Techniques
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Chemicals and reagents used in the study were obtained from Millipore-Sigma, (USA), and SRL (India). Bacterial culture media was from HiMedia (India). Restriction enzymes and DNA modifying enzymes were purchased from New England Biolabs (USA). Culture media were from Sera Laboratory International Limited (UK) and Lonza (Switzerland). Fetal bovine serum and PenStrep were from GIBCO (USA). Antibodies were from Santa Cruz Biotechnology (USA). γP32-ATP was purchased from BRIT (India).
3
Cell Culture Media Components Procurement
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Bacterial culture media was purchased from HiMedia, Mumbai. All cell culture media components, Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS), and antibiotics were purchased from HiMedia Laboratories, Mumbai. The cell culture assay reagents, trypsin, MTT, ethidium bromide, rhodamine-123, acridine orange, PBS, low-melting point agarose, EDTA, tris buffer, ammonium persulfate, and ethyl acetate, were purchased from Sigma Aldrich, India. The TUNEL APO-BrdU apoptosis kit was procured from Invitrogen.
4
Purification of Recombinant Proteins
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The molecular biology kits and Ni-NTA agarose were purchased from Qiagen, CA, USA. The dNTPs and enzymes were purchased from New England Biolabs, MA, USA. All other reagents and chemicals were of the highest purity available and were purchased either from Sigma-Aldrich Chemical Company, St. Louis, MO, USA or Sisco Research Laboratories, Mumbai, India. Bacterial culture media was purchased from Himedia Laboratories, Mumbai, India.
5
Purification of Recombinant Proteins
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Restriction enzymes and deoxynucleotide triphosphates (dNTPs) were indented from New England Biolabs (NEB), USA. All other chemicals and reagents were purchased from the Sisco Research Laboratories (SRL), Sigma-Aldrich Chemical Company, Mumbai, India, and USA, and were of the highest purity available. The Nickel-Nitrilotriacetic Acid (Ni–NTA) Agarose matrix and molecular biology kits were purchased from Qiagen, CA, USA. Bacterial culture media were indented from Himedia Laboratories, Mumbai, India.
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