Ketek

All reagents were purchased from Sigma-Aldrich (Oakville, Ontario, Canada) unless otherwise specified. Acetyl-coenzyme A (CoA) was purchased from BioShop (Burlington, Ontario, Canada). Organic solvents were purchased from Fisher Scientific (Ottawa, Ontario, Canada). Telithromycin was purified from the pharmaceutical formulation Ketek (400 mg, Sanofi-Aventis US). Briefly, pills were crushed in a mortar and pestle, dissolved in acetonitrile at 40 °C, passed through an Amicon Ultra-15 10 kDa centrifugal filter unit and lyophilized. Linopristin and flopristin were gifts from AstraZeneca.

All buffers and salts were purchased from BioShop (Burlington, ON, Canada) unless otherwise specified. Macrolide antibiotics, GTP, pyruvate kinase: lactate dehydrogenase (PK:LDH), phosphoenolpyruvate, and NADH were purchased from Sigma-Aldrich (Oakville, ON, Canada). Molecular biology kits and organic solvents were purchased from Fisher Scientific (Ottawa, ON, Canada). Telithromycin was purified from the drug formulation Ketek (400 mg, Sanofi-Aventis US)^{36 (link)}.