Serum was collected and stored at − 80 °C until analysed. Fifty microliters of serum were analyzed for IL-1α, G-CSF, IL-10, IL-17A, IL-1β, IL-6, TNFα, IL-4, GM-CSF, IFNγ, IL-2, IL-5, IL-13, IL-12p70, Eotaxin, GRO-α, IP-10, MCP-1, MCP-3, MIP-1α, MIP-2, and Rantes (Rat Cytokine & Chemokine 22-plex ProcartaPlex Panel, Invitrogen, Catalog #EPX220-30122-901) according to manufacturer’s instructions using a Luminex 200 instrument (Thermo Fisher Scientific, Waltham MA).
22 plex procartaplex panel
Manufactured by Thermo Fisher Scientific
The 22-plex ProcartaPlex Panel is a multiplexed immunoassay kit designed for the simultaneous detection and quantification of 22 different analytes in a single sample. It is a tool for researchers to assess multiple biological targets in a streamlined manner.
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Lab products found in correlation
2 protocols using 22 plex procartaplex panel
1
Cytokine and Chemokine Profiling in Serum
2
Cytokine and Chemokine Profiling in Rat Sera
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Serum collected at time of necropsy was stored at − 80 °C until analysed. Samples from all gestation (GD11 and GD18) and treatment (control and infected) groups were analysed at the same time within the same assay. Briefly, 50 µL of serum were analyzed for IL-1α, G-CSF, IL-10, IL-17A, IL-1β, IL-6, TNFα, IL-4, GM-CSF, IFNγ, IL-2, IL-5, IL-13, IL-12p70, Eotaxin, GRO-α, IP-10, MCP-1, MCP-3, MIP-1α, MIP-2, and Rantes (Rat Cytokine & Chemokine 22-plex ProcartaPlex Panel, Invitrogen, Catalog #EPX220-30122-901) as per the manufacturer’s instructions. Samples were analysed with a Luminex 200 instrument (Thermo Fisher Scientific, Waltham MA).
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