C2C12 cells were obtained from ATCC and cultured in Dulbecco's modified Eagle Medium (DMEM) supplemented with 10% FBS (Microgem), 2 mM glutamine, 50 i.u. penicillin, and 50 μg/ml streptomycin (proliferating medium). C2C12 TET-ON D2 cells were cultured in DMEM supplemented with 10% tetracycline-free Fetal Bovine Serum (FBS) (Clontech, Mountain View, CA, USA), 100 μg/ml geneticin (Biowest, Nuaillé, France) and 0.8 μg/ml puromycin (Invitrogen, Carlsbad, CA, USA). For studies in proliferative conditions (PROL), cells were grown at 40–50% confluence and treated with the indicated amounts of doxycycline for 16, 24 or 48 h as indicated. For experiments in differentiation conditions (DIFF), cells were grown at 60%–70% confluence, treated with doxycycline and then induced to differentiate in Differentiation Medium (DMEM with 2% horse serum, insulin 10 μg/ml and transferrin 5 μg/ml). D2 expression was turned on by 2 μg/ml doxycycline (Clontech, Mountain View, CA, USA). In some experiments, TH (T3 and T4, Sigma-Aldrich S. Louis, Missouri, USA) were added in culture medium at a 30 nM final concentration or removed from the FBS by charcoal absorption (Larsen, 1972).
Geneticin
Manufactured by Biowest
Sourced in United States, France
Geneticin is a broad-spectrum antibiotic commonly used as a selective agent in cell culture applications. It inhibits protein synthesis in eukaryotic cells, making it effective for selecting and maintaining cell lines that have been transfected with a gene conferring resistance to this antibiotic.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Biowest (2 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Takara Bio (1 mentions)
Doxycycline, by Takara Bio (1 mentions)
Fetal bovine serum (fbs), by Microgem (1 mentions)
Gelatin type a, by Merck Group (1 mentions)
Penicillin streptomycin, by Biowest (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Dulbecco phosphate buffered saline (dpbs), by Merck Group (1 mentions)
Resazurin sodium salt, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Carl Roth (1 mentions)
Roti immunoblock, by Carl Roth (1 mentions)
Calcium chloride, by Carl Roth (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Sodium alginate, by Carl Roth (1 mentions)
Dmem f12, by Biowest (1 mentions)
Trisodium citrate dihydrate, by Carl Roth (1 mentions)
Nih3t3, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
3 protocols using geneticin
1
Doxycycline-Induced C2C12 Differentiation
2
Endothelial Cell Culture in 3D Scaffold
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Cell culture medium DMEM Low Glucose, DMEM F-12, Penicillin/Streptomycin, geneticin and FBS were obtained from Biowest (Nuaillé, France). Cell culture media MCDB 131 and rhEGF were purchased from PAN Biotech (Aidenbach, Germany). Hydrocortison, ascorbic acid, DAPI, DPBS, propidium iodide and gelatin type A (300 bloom) were obtained from Merck (Darmstadt, Germany). Heparin sodium salt was purchased from Serva (Heidelberg, Germany). Sodium alginate, calcium chloride, tri-sodium citrate-dihydrate, silicon oil, Roti-Immunoblock and L-glutamine were ordered from Carl Roth (Karlsruhe, Germany). Bovine pituitary extract was obtained from Thermo Fisher/Life Technologies (Darmstadt, Germany). Resazurin sodium salt was purchased at Alfa Aesar (War Hill, GA, USA). CD31 antibody was purchased from Becton Dickinson (Heidelberg, Germany). Calcein blue AM was purchased from AAT Bioquest (Sunnyvale, CA, USA). Cyclo-Olefin Copolymer COC 5013 granulat (TOPAS Advanced Polymers GmbH, Frankfurt am Main, Germany) was a friendly gift from 3D Schilling GmbH. COC slides were purchased from Microfluidic Chipshop (Jena, Germany). Microbial transglutaminase (mTGM) was obtained from Ajinomoto (Hamburg, Germany).
3
Cell Line Cultivation for FGFR1-IIIc Study
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NIH3T3 (CRL-1658) cell line was purchased from American Type Culture Collection (Manassas, VA, USA). U2OS stably transfected with FGFR1-IIIc (U2OS-R1) was a kind gift from Dr. Ellen M. Haugsten from the Department of Molecular Cell Biology, Institute for Cancer Research (Oslo University Hospital). NIH3T3 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) from Thermo Fisher Scientific, containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin and 100 µg/mL streptomycin (BioWest, Nuaillé, France). U2OS-R1 cells were cultivated in DMEM from BioWest supplemented with 10% fetal bovine serum and antibiotics (100 U/mL penicillin, 100 µg/mL streptomycin and 1mg/mL geneticin—BioWest). All cell lines were cultured in a humidified incubator at 37 °C in 5% CO2 atmosphere. Cells were seeded into tissue culture plates the day preceding the start of the experiments.
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