A transfected Chinese hamster ovary (CHO), Hela, a murine interleukin-3 dependent pro-B (Ba/F3), PC12, and rat basophil leukemia cell lines were obtained from Eurofins Scientific (Eurofins-Cerep, Le Bois I’Eveque, France). Buffers—Dulbecco’s modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS) buffer—were purchased from Invitrogen (Carlsbad, CA, USA). The reference agonists: 5′-N-ethylcarboxamidoadenosine (NECA), epinephrine bitartrate, epinephrine, DPDPE, CP 55940, linoleic acid, GLP-1(7–37, arginine vasopressin (AVP), and serotonin, and antagonists: ZM 241385, RX-821002, rauwolscine, naltriben mesylate, AM 281, exendin-3(9–39), [d(CH2)51, Tyr (Me)2]-AVP, (S)-WAY-100635, and GR55562) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents purchased from Merck and Fluka were of the highest available grade unless otherwise stated.
Dulbecco s modified eagle medium dmem buffer
Manufactured by Thermo Fisher Scientific
Sourced in United States
Dulbecco's modified Eagle medium (DMEM) is a common cell culture medium that provides a balanced salt solution and a source of energy, vitamins, and amino acids to support the growth and maintenance of cells in vitro. It is a widely used buffer for various cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes buffer, by Thermo Fisher Scientific (5 mentions)
Hank s balanced salt solution hbss buffer, by Thermo Fisher Scientific (4 mentions)
S way 100635, by Merck Group (3 mentions)
L 733 060, by Merck Group (2 mentions)
Epinephrine bitartrate, by Merck Group (1 mentions)
Rauwolscine, by Merck Group (1 mentions)
Zm241385, by Merck Group (1 mentions)
5 n ethylcarboxamidoadenosine neca, by Merck Group (1 mentions)
Cp55940, by Merck Group (1 mentions)
Glp 1 7 37, by Merck Group (1 mentions)
Rx 821002, by Merck Group (1 mentions)
Am281, by Merck Group (1 mentions)
Arginine vasopressin avp, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Dpdpe, by Merck Group (1 mentions)
Epinephrine, by Merck Group (1 mentions)
Clozapine, by Merck Group (1 mentions)
Acetylcholine chloride, by Merck Group (1 mentions)
Dopamine, by Merck Group (1 mentions)
Serotonin, by Merck Group (1 mentions)
5 protocols using dulbecco s modified eagle medium dmem buffer
1
Cell Lines and Reagents for Bioassays
2
Transfected Cell Lines and Reference Agonists/Antagonists
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The transfected Chinese hamster ovary (CHO) cells, rat basophil leukemia (RBL) cells, U373 cells and BA/F3 cells were obtained from Eurofins Scientific (Le Bois I’Eveque, France). Various buffers namely Hank’s balanced salt solution (HBSS) buffer, Dulbecco’s modified Eagle medium (DMEM) buffer and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer were obtained from Invitrogen (Carlsbad, CA, USA). The reference agonists and/or antagonists atropine sulphate salt, acetylcholine chloride, [Sar9, Met(O2)11]-Substance P, [Arg8]-vasopressin (AVP), (+)butacamol, clozapine, dopamine, [d(CH2)5 1, Tyr(Me)2]-AVP, serotonin, SCH 2330, L-733,060, (S)-WAY-100635 and 3-isobutyl-1-methylxanthine (IBMX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the other chemicals and reagents used were purchased from E. Merck, Fluka (Rupert-Mayer-Str., Munich, Germany), and Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated, and were of highest grade available.
3
Characterization of Cell Lines for Drug Screening
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Human endogenous (U373MG cells), murine interleukin-3 dependent pro-B (Ba/F3) and a transfected Chinese hamster ovary (CHO) cell lines were obtained from Eurofins Scientific (Le Bois I’Eveque, France). Buffers—Dulbecco’s modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer and Hank’s balanced salt solution (HBSS) buffer—were purchased from Invitrogen (Carlsbad, CA, USA). The reference agonists dopamine, [Sar9, Met(O2)11]-SP, serotonin and arginine vasopressin, and antagonists (+) butaclamol, L 733060, (S)-WAY-100635, and [d(CH2)51,Tyr(Me)2]-AVP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents were purchased from Merck and Fluka, unless otherwise stated and were of highest available grade.
4
Monoamine Oxidase Enzyme Assay Protocol
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We purchased Human recombinant
monoamine oxidase (hMAO) isozymes, deprenyl, clorgyline-HCl, safinamide
mesylate salt, emodin, and dimethyl sulfoxide (DMSO) from Sigma-Aldrich
Co. (St Louis, MO, USA). The MAO-GloTM assay kit was purchased from
Promega (Promega Corporation, Madison, WI, USA). A transfected Chinese
hamster ovary (CHO) cell line was purchased from Eurofins Scientific
(Le Bois I’Eveque, France). Buffers—Dulbecco’s
modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS)
buffer from Invitrogen (Carlsbad, CA, USA). The reference agonist
arginine vasopressin and antagonist were purchased from Sigma-Aldrich
(St. Louis, MO, USA). All the drugs, chemicals, and reagents were
of the highest grade available and were purchased from Merck (Darmstadt,
Germany) and Fluka (Buchs, Switzerland), unless otherwise stated.
monoamine oxidase (hMAO) isozymes, deprenyl, clorgyline-HCl, safinamide
mesylate salt, emodin, and dimethyl sulfoxide (DMSO) from Sigma-Aldrich
Co. (St Louis, MO, USA). The MAO-GloTM assay kit was purchased from
Promega (Promega Corporation, Madison, WI, USA). A transfected Chinese
hamster ovary (CHO) cell line was purchased from Eurofins Scientific
(Le Bois I’Eveque, France). Buffers—Dulbecco’s
modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer, and Hank’s balanced salt solution (HBSS)
buffer from Invitrogen (Carlsbad, CA, USA). The reference agonist
arginine vasopressin and antagonist were purchased from Sigma-Aldrich
(St. Louis, MO, USA). All the drugs, chemicals, and reagents were
of the highest grade available and were purchased from Merck (Darmstadt,
Germany) and Fluka (Buchs, Switzerland), unless otherwise stated.
5
Isolation and Identification of Bromophenols
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We isolated three bromophenols, namely, 2,3,6tribromo-4,5-dihydroxybenzyl alcohol (1), 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (2), and bis-(2,3,6tribromo-4,5-dihydroxybenzyl) ether (3) (Fig. 1), from the leafy thalli of Symphyocladia latiuscula (Harvey) Yamada and identified as described in our recent report [63] . We estimated the purity of the test compounds to be >98% as evidenced by proton and carbon NMR spectra. We obtained transfected Chinese hamster ovary (CHO) cells from Eurofins Scientific (Le Bois I'Eveque, France). We obtained Hank's balanced salt solution (HBSS) buffer, Dulbecco's modified Eagle medium (DMEM) buffer, and 4-(2hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer from Invitrogen (Carlsbad, CA, USA). We purchased reference drugs CCK-8s and YM022 from Sigma-Aldrich (St. Louis, MO, USA).
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