Human jurkat cells

Manufactured by American Type Culture Collection

Human Jurkat cells are a type of immortalized T lymphocyte cell line derived from a patient with T cell leukemia. These cells are widely used in research as a model for T cell signaling and activation.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions) Rpmi 1640 medium, by American Type Culture Collection (1 mentions) Mtesr1, by STEMCELL (1 mentions) Human ad293 cells, by Agilent Technologies (1 mentions) Airway epithelial cell basal medium, by American Type Culture Collection (1 mentions) Bronchial epithelial cell growth kit, by American Type Culture Collection (1 mentions) Human igg4, by BioLegend (1 mentions) Alemtuzumab, by Sanofi (1 mentions) Rat igg2a, by BioLegend (1 mentions) Rituximab, by Roche (1 mentions) Pcr mycoplasma test kit 1 c, by PromoCell (1 mentions) Cyclosporine a, by Merck Group (1 mentions)

Close spelling variants of this product

Jurkat (389 citations) Jurkat cells (116 citations) Jurkat human T cells (3 citations) Human Jurkat T cells (Clone E6-1) (2 citations) Jurkat cells (human T cell) and FADD-deficient (FADD−/−) Jurkat cells (2 citations) Jurkat human T cell leukemia cells (2 citations) Human Jurkat T leukemia cells (2 citations)

4 protocols using human jurkat cells

Culturing Human Cell Lines for Research

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Human fibroblast cells (Cat# CRL2522) were acquired from American Type Culture Collection (ATCC, Manassas, VA). Cells were cultured with RPMI-1640 Medium (Cat# 11875119; ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS; Cat# FBS1824-001, Nucleus Biologics, San Diego, CA).
Human AECs were obtained from ATCC (Cat# PCS-300-010) and cultured with Airway Epithelial Cell Basal Medium (Cat# PCS-300-030, ATCC) supplemented with Bronchial Epithelial Cell Growth Kit (Cat# PCS-300-040, ATCC).
Human iPSC cells (Cat# ACS-1030, ATCC) were cultured in feeder-free condition in mTeSR1 (Cat# 85850, StemCell Technologies, Vancouver, Canada) on Matrigel (Cat# 354277, Corning Inc, Corning, NY)-coated cultureware surface.
Human Ad293 cells (Cat# 240085, Agilent, Santa Clara, CA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cat# 11995-065, ThermoFisher Scientific) supplemented with 10% fetal bovine serum.
Human CD34⁺ HSCs (Cat# PCS-800-012, ATCC) were cultured in Steriflip-filtered StemSpan SFEM II medium (Cat# 09655, StemCell Technologies) supplemented with SCF (100 ng/ml), TPO (100 ng/ml), Flt3L (100 ng/ml), IL-6 (100 ng/ml), UM171 (35 nM), 20 mg/ml streptomycin, 20 U/ml penicillin, and SR1 (StemRegenin1; 0.75 μM).
Human Jurkat cells (Cat# TIB-152, ATCC) were cultured in RPMI-1640 Medium (Cat# 30-2001, ATCC) supplemented with 10% FBS.

Ma L., Ruan J., Song J., Wen L., Yang D., Zhao J., Xia X., Chen Y.E., Zhang J, & Xu J. (2020). MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Nature Communications, 11, 6082.

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Evaluation of Humanized Anti-Clever-1 Antibody

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The humanized anti–Clever-1 antibody bexmarilimab was generated and produced by Abzena. The anti–Clever-1 parental antibody 3–372 (InVivo Biotech), the humanized anti–Clever-1 antibody FP-1304 without the L248E mutation (Antitope Ltd), rituximab (Roche), alemtuzumab (Genzyme), cetuximab (Merck Healthcare KGaA), and the isotype control antibodies human IgG4 (Biolegend) and rat IgG2a (Biolegend) were used as references and controls as indicated in the respective method descriptions.
KG-1 human acute myelogenous leukemia and human Jurkat cells were purchased from ATCC and NS0 murine myeloma cells from the European Collection of Authenticated Cell Cultures (ECACC). The cell lines were routinely tested for Mycoplasma with PCR Mycoplasma Test Kit I/C (Promocell). Cell line authentication was not performed as the cells were used from a master cell bank (<5 passages) created from the original vial.

Hollmén M., Maksimow M., Rannikko J.H., Karvonen M.K., Vainio M., Jalkanen S., Jalkanen M, & Mandelin J. (2022). Nonclinical Characterization of Bexmarilimab, a Clever-1–Targeting Antibody for Supporting Immune Defense Against Cancers. Molecular Cancer Therapeutics, 21(7), 1207-1218.

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Prostate Cancer Cell Lines Response

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Two human prostate adenocarcinoma cell lines (LNCaP cells and PC-3 cells) and human Jurkat cells (American Type Culture Collection, Rockville, MD) were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum. The response to PSMA for LNCaP and PC-3 cells was positive and negative, respectively, which has been confirmed in the previous study [15 (link)].

Meng P., Dong Q.C., Tan G.G., Wen W.H., Wang H., Zhang G., Wang Y.Z., Jing Y.M., Wang C., Qin W.J, & Yuan J.L. (2017). Anti-tumor effects of a recombinant anti-prostate specific membrane antigen immunotoxin against prostate cancer cells. BMC Urology, 17, 14.

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Cell Culture and Compound Preparation

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Human A549 and 293T cells, owl monkey kidney OMK cells and canine Cf2Th cells obtained from the American Type Culture Collection (ATCC) were grown at 37 °C in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS and 1% penicillin-streptomycin. Human Jurkat cells obtained from the American Type Culture Collection (ATCC) were grown at 37 °C in Roswell Park Memorial Institute Medium (RPMI) supplemented with 10% FBS and 1% penicillin-streptomycin. PF74 and cyclosporine A were obtained from Sigma Aldrich (SML0835). They were dissolved in dimethyl sulfoxide (DMSO) to create a stock of 10 mM.

Selyutina A., Simons L.M., Kirby K.A., Bulnes-Ramos A., Hu P., Sarafianos S.G., Hultquist J.F, & Diaz-Griffero F. (2022). TRIM5α Restriction of HIV-1-N74D Viruses in Lymphocytes Is Caused by a Loss of Cyclophilin A Protection. Viruses, 14(2), 363.

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