Icaritin with a purity of 99.5% was obtained from Shenogen Pharma Co (Beijing, China). icaritin preparation in vitro and in vivo was described previously [10 (link)]. Autophagy inhibitors chloroquine (Cq), ammonium chloride (NH4Cl) and 3-methyaldenine (3-MA) were obtained from Sigma-Aldrich (St. Louis, MO). Compound 13 was from Dr. Zhao's group [43 (link)]. MHY1485 was purchased from Biotools (Shanghai, China). Antibodies utilized in this study were all obtained from Cell Signaling Tech (Santa Cruz, CA).
3 methyaldenine 3 ma
Manufactured by Merck Group
Sourced in Sao Tome and Principe
3-methyaldenine (3-MA) is a chemical compound used in laboratory research. It is a white crystalline solid. 3-MA is commonly used as a reagent in various scientific experiments and analyses, but its specific core function should not be extrapolated upon without further context.
Automatically generated - may contain errors
Lab products found in correlation
Puromycin, by Merck Group (3 mentions)
Chloroquine cq, by Merck Group (2 mentions)
Erk1 2 antibody, by Santa Cruz Biotechnology (1 mentions)
Total s6k1, by Cell Signaling Technology (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Z devd fmk, by Merck Group (1 mentions)
4 protocols using 3 methyaldenine 3 ma
1
Icaritin Autophagy Modulators Protocol
2
GSK621 Autophagy Regulation Assay
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GSK621 was purchased from Gao-Chem (Shanghai, China). H2O2, puromycin, 3-methyaldenine (3-MA) and chloroquine (Cq) were purchased from Sigma Chemicals (St. Louis, MO). Anti-AMPKα1, acetyl-CoA carboxylase (ACC) and Beclin-1, autophagy-related homologue 5 (ATG-5), light chain 3B-II (LC3B-II), p62 and Erk1/2 antibodies were purchased from Santa Cruz Biotech (Santa Cruz, CA). Antibodies against phospho(p)-AMPKα1 (Thr 172), p-ACC (Ser 79), p-S6K1 (Thr-389), p-S6 (Ser-235/236) and total S6K1 were obtained from Cell Signaling Tech (Denver MA).
3
Immunological Assays with Molecular Inhibitors
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Kaempferol, 3-methyaldenine (3-MA) and puromycin were purchased from Sigma-Aldrich (Sigma, St. Louis, MO). C8 ceramide was a gift from Dr. Wang [27 (link)]. The anti-MAGEA6 antibody was obtained from Abcam (Shanghai, China). All the other antibodies were provided by Cell Signaling Tech Co. (Denver MA). The reagents for cell culture were from Gibco BRL (Suzhou, China).
4
Investigating Apoptosis and Autophagy
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CC-115 was provided by MCE China (Shanghai, China). Puromycin, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk as well as the autophagy inhibitors chloroquine (Cq) and 3-methyaldenine (3-MA) were obtained from Sigma-Aldrich (St. Louis, MO). The antibodies of this study were provided by Cell Signaling Tech (Shanghai, China). Cell culture reagents were purchase from Gibco (Suzhou, China).
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