Galangin (3,5,7-trihydroxyflavone) and primary antibodies against ERK, phospho-ERK, GCLC, and GSS were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and primary antibody against actin were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Buthioninesulfoximine (BSO) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Primary antibodies against TATA-binding protein (TBP) and phospho-Nrf2 were purchased from Abcam (Cambridge, MA, USA). Primary antibodies against Nrf2, AKT, and phospho-AKT were purchased from Cell Signaling Technology (Beverly, MA, USA). Cell TrackerTM Blue CMAC was purchased from Molecular Probes (Eugene, OR, USA). All other chemicals and reagents were of analytical grade.
Primary antibodies against nrf2
Manufactured by Cell Signaling Technology
Sourced in United States
Primary antibodies against Nrf2. These antibodies specifically recognize the Nrf2 protein, a transcription factor that plays a key role in the cellular response to oxidative stress.
Automatically generated - may contain errors
Lab products found in correlation
Primary antibody against actin, by Merck Group (1 mentions)
Buthionine sulfoximine bso, by Cayman Chemical (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Phospho erk, by Santa Cruz Biotechnology (1 mentions)
Phospho nrf2, by Abcam (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Tcs sp2 confocal microscope, by Leica (1 mentions)
Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions)
Labtek 2 chambers, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
2 protocols using primary antibodies against nrf2
1
Galangin's Modulation of Cellular Antioxidant Pathways
2
NRF2 Translocation Assay in HREC
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To perform the NRF2 translocation analyses, HREC were seeded in fibronectin-coated LabTek II chambers (Nalge Nunc International, Rochester, NY, USA) and treated with Pter 5 uM (DMSO as the vehicle was used at a concentration of 0.1%) 24 h later. Cells were incubated for 0, 5, 6 or 8 h and were washed with PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) for 30 min. Then cells were incubated with 1% Triton X-100 in PBS for 10 min to permeabilise cell membranes, followed by incubation with 3% bovine serum albumin (BSA) and 5% FBS in PBS for 1 h. Cells were incubated with 1: 250 dilution of primary antibodies against NRF2 (Cell Signaling, Danvers, MA, USA) overnight at 4 °C, followed by the Alexa Fluor® secondary antibody (Fisher Scientific, Madrid, Spain) for 1 h at room temperature in the dark. Nuclei were stained with 4,6-diamidino-2-phenyindole, dihydrochloride (DAPI) (Fisher Scientific, Madrid, Spain) and then examined under a Leica TCS SP2 confocal microscope (Leica, Wetzlar, Germany).
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