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Lactate dehydrogenase

Manufactured by Randox
Sourced in United Kingdom

Lactate dehydrogenase is an enzyme that catalyzes the interconversion of lactate and pyruvate. It is a key enzyme involved in the glycolytic pathway and plays a crucial role in cellular energy metabolism.

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2 protocols using lactate dehydrogenase

1

Mouse Inflammation and Biomarker ELISA Protocol

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Duoset mouse Lcn2 and CXCL1 (KC) ELISA kit, and anti-mouse Lcn2 antibody were obtained from R&D Systems. Aspartate transaminase (AST), alanine transaminase (ALT) triglycerides (TG), cholesterol (CHOL), lactate dehydrogenase (LDH) and creatinine kits were procured from Randox Laboratories. Glucometer and glucose test strips were obtained from Nova Max. Iron atomic absorption (AA) standard was purchased from RICCA Chemical Company. Ferrous sulfate was obtained from Alfa Aesar. SYBR® Green mix and qScript cDNA synthesis kit was procured from Quanta Biosciences. Bleomycin sulfate was procured from Biotang. Reagent grade Dextran Sulfate Sodium salt (DSS, reagent grade, M.W. 36–50 kDa, Ref = 160110) was purchased from MP Biomedicals. Mouse recombinant (rec)-Lcn2 was obtained from Cell Signaling and human rec-NGAL was acquired from R&D Systems; both of which are free from endotoxin, siderophore, and iron. All other fine chemicals used in present study were reagent grade and procured from Sigma.
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2

Investigating Hepatoprotective Effects

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Lead acetate, TUR, and Vit-C were purchased from Sigma Chemical Co. (Sigma, St.
Louis, Missouri, USA). Commercial kits for the assay of alanine transaminase
(ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) were purchased
from (Randox Laboratories Ltd, Crumlin, United Kingdom). Primary antibodies for
BCL-2-associated X (Bax) and B-cell CLL/lymphoma 2 (Bcl-2) were purchased from
(Santa Cruz Biotechnology, Santa Cruz, California). Secondary antibodies were
purchased from Sigma-Aldrich.
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