Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences, Inc., San Diego, CA, USA) according to the manufacturer’s instructions. In brief, hADSCs (1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING, Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture, which was then incubated on ice for 30 min. After washing the cells with Brilliant Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), FACSs measurement was carried out. The following primary antibodies was used: APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences); FITC anti-human CD90.2 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
Pe cy7 anti human cd45 antibody
Manufactured by BioLegend
Sourced in United States
The PE/Cy7 anti-human CD45 Antibody is a fluorescence-labeled monoclonal antibody that specifically binds to the CD45 antigen on human cells. CD45 is a protein tyrosine phosphatase that is expressed on the surface of all human leukocytes.
Automatically generated - may contain errors
Lab products found in correlation
Novocyte flow cytometer, by Agilent Technologies (2 mentions)
Perfusion solution, by Corning (2 mentions)
Pe cy7 mouse igg1 κ isotype ctrl antibody, by BioLegend (2 mentions)
Apc mouse anti human cd29, by BD (2 mentions)
Brilliant stain buffer, by BD (2 mentions)
Fitc mouse igg1 κ isotype ctrl antibody, by BioLegend (1 mentions)
2 protocols using pe cy7 anti human cd45 antibody
1
Flow Cytometry Analysis of hADSCs
2
Characterization of hMSC-AT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell flow cytometry was performed using a NovoCyte® Flow Cytometer (ACEA Biosciences,
Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, hMSC-ATs
(1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING,
Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture,
which was then incubated on ice for 30 minutes. After washing the cells with Brilliant
Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence activated cell
sorting (FACS) measurement was carried out. The following primary antibodies were used:
APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype
Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences, Franklin Lakes, NJ, USA); FITC
anti-human CD90 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human
CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody,
and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
Inc., San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, hMSC-ATs
(1 × 105 cells) were mixed into 0.5 mL of Perfusion Solution (CORNING,
Manassas, VA, USA). Each antibody (1/100 of the volume) was added to the cell admixture,
which was then incubated on ice for 30 minutes. After washing the cells with Brilliant
Stain Buffer (BD Biosciences, Franklin Lakes, NJ, USA), fluorescence activated cell
sorting (FACS) measurement was carried out. The following primary antibodies were used:
APC Mouse Anti-Human CD29, BV421 Mouse Anti-Human CD44, BV421 Mouse IgG2b κ Isotype
Control, APC Mouse IgG1 κ Isotype Control (BD Biosciences, Franklin Lakes, NJ, USA); FITC
anti-human CD90 (Thy1) Antibody, FITC Mouse IgG1 κ Isotype Ctrl Antibody, PerCP anti-human
CD34 Antibody, PerCP Mouse IgG1 κ Isotype Ctrl Antibody, PE/Cy7 anti-human CD45 Antibody,
and PE/Cy7 Mouse IgG1 κ Isotype Ctrl Antibody (BioLegend, Inc., San Diego, CA, USA).
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