Liquid chromatography for all LC-MS/MS runs was performed on an EASY-nLC 1000 Liquid Chromatography system (Thermo Scientific) coupled to the spectrometers via modified NanoFlex sources (Thermo scientific). Peptides were loaded onto 250-mm x 75-μm PicoFrit (C18 2 μm medium) analytical columns (New Objective) at a maximum pressure of 800 bar. Solutions A and B for the UPLCs were 0.1% formic acid in water and acetonitrile, respectively. Samples were loaded in 0.1% formic acid in water to maximize retention of highly hydrophilic peptides. Gradients varied slightly in length (90 to 150 min) and mixture, and may be extracted from the respective raw files. In general they incorporated a linear gradient from very low or zero %B to 20 or 30% for 65-100 minutes, followed by a steeper phase and a wash. This length of gradient was maintained despite the relative simplicity of the protein mixture in order to improve the resolution and identification of as many modified peptide forms as possible, including those of low abundance.
Picofrit c18 2 μm medium analytical columns
Manufactured by New Objective
PicoFrit (C18 2 μm medium) analytical columns are high-performance liquid chromatography (HPLC) columns designed for analytical applications. The columns feature a 2 μm particle size C18 stationary phase material.
Automatically generated - may contain errors
3 protocols using picofrit c18 2 μm medium analytical columns
1
Optimized LC-MS/MS Peptide Analysis
2
Peptide Separation and Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid chromatography for all LC-MS/MS runs was performed on an EASY-nLC 1000 Liquid Chromatography system (Thermo Scientific) coupled to the spectrometers via modified NanoFlex sources (Thermo scientific). Peptides were loaded onto 250-mm x 75-μm PicoFrit (C18 2 μm medium) analytical columns (New Objective) at a maximum pressure of 800 bar. Solutions A and B for the UPLCs were 0.1% formic acid in water and acetonitrile, respectively. Samples were loaded in 0.1% formic acid in water to maximize retention of highly hydrophilic peptides. Gradients varied slightly in length (90 to 150 min) and mixture, and may be extracted from the respective raw files. In general they incorporated a linear gradient from very low or zero %B to 20 or 30% for 65-100 min, followed by a steeper phase and a wash. This length of gradient was maintained despite the relative simplicity of the protein mixture in order to improve the resolution and identification of as many modified peptide forms as possible, including those of low abundance.
3
Optimized LC-MS/MS Peptide Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid chromatography for all LC-MS/MS runs was performed on an EASY-nLC 1000 Liquid Chromatography system (Thermo Scientific) coupled to the spectrometers via modified NanoFlex sources (Thermo scientific). Peptides were loaded onto 250-mm x 75-μm PicoFrit (C18 2 μm medium) analytical columns (New Objective) at a maximum pressure of 800 bar. Solutions A and B for the UPLCs were 0.1% formic acid in water and acetonitrile, respectively. Samples were loaded in 0.1% formic acid in water to maximize retention of highly hydrophilic peptides. Gradients varied slightly in length (90 to 150 min) and mixture, and may be extracted from the respective raw files. In general they incorporated a linear gradient from very low or zero %B to 20 or 30% for 65-100 minutes, followed by a steeper phase and a wash. This length of gradient was maintained despite the relative simplicity of the protein mixture in order to improve the resolution and identification of as many modified peptide forms as possible, including those of low abundance.
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