Acquity tq triple quadrupole mass spectrometer

Manufactured by Waters Corporation

Sourced in United States

The Acquity TQ triple quadrupole mass spectrometer is a laboratory instrument designed for sensitive and selective detection and quantification of compounds. It utilizes a triple quadrupole configuration to perform tandem mass spectrometry analysis, enabling high-performance separation and detection of target analytes.

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Lab products found in correlation

996 photodiode array detector, by Waters Corporation (1 mentions) 2695 high performance liquid chromatography system, by Waters Corporation (1 mentions) Kinetex c18, by Phenomenex (1 mentions) Alliance 2695 high performance liquid chromatography, by Waters Corporation (1 mentions)

3 protocols using acquity tq triple quadrupole mass spectrometer

Optimized MRM Analysis of Pesticide Residues

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One precursor and the product ion(s) for each pesticide of interest were selected by first running the single MS full scan mode on a Waters 2695 high-performance liquid chromatography system coupled with a UV detector (Waters 996 photodiode array detector) and a Waters Acquity TQ triple quadrupole mass spectrometer (MS/MS) (Waters TQ Detector, Acquity ultra-performance LC). Subsequently, the product ion scan mode was performed. Within the Waters Empower 3 Chromatogaphy software, the AutoTune was performed on each individual analyte using the obtained precursor ion and the product ions. The MRM experimental optimal parameters were selected from the generated report and the precursor scan was used to determine retention time.

Hooper S.E., Amelon S.K, & Lin C.H. (2022). Development of an LC-MS/MS Method for Non-Invasive Biomonitoring of Neonicotinoid and Systemic Herbicide Pesticide Residues in Bat Hair. Toxics, 10(2), 73.

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Quantification of Compounds in Wastewater

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The compounds identified through untargeted analysis were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS analyses were performed using an HPLC system (Water Alliance 2695, Water Co., Milford, MA, United States) coupled with a Waters Acquity TQ triple quadrupole mass spectrometer operated in negative and positive electrospray ionization modes (SI. 5, Supplementary Information). Concentrations of the compounds found in wastewater extracts were determined based on a calibration curve for each analyte generated using standards of these compounds at 8 concentrations (0.01, 0.05, 0.1, 0.5, 1.25, 2.5, 5, 10 mg/L) in triplicate. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to assess the sensitivity of the analytical method. For each compound, the signal-to-noise ratios of three and ten were employed to calculate LOD and LOQ, respectively.

Bayati M., Hsieh H.Y., Hsu S.Y., Li C., Rogers E., Belenchia A., Zemmer S.A., Blanc T., LePage C., Klutts J., Reynolds M., Semkiw E., Johnson H.Y., Foley T., Wieberg C.G., Wenzel J., Lyddon T., LePique M., Rushford C., Salcedo B., Young K., Graham M., Suarez R., Ford A., Lei Z., Sumner L., Mooney B.P., Wei X., Greenlief C.M., Johnson M.C, & Lin C.H. (2022). Identification and quantification of bioactive compounds suppressing SARS-CoV-2 signals in wastewater-based epidemiology surveillance. Water Research, 221, 118824.

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Quantification of Neurotransmitter Metabolites

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The quantification of 5-HIAA, creatinine, caffeine, and paraxanthine was performed by a Waters Alliance 2695 High Performance Liquid Chromatography (HPLC) system coupled with Waters Acquity TQ triple quadrupole mass spectrometer (MS/MS). The analytes were separated using a Phenomenex (Torrance, CA) Kinetex C18 (100mm × 4.6 mm; 2.6 μm particle size) reverse-phase column. The mobile phase consisted of (A)10 mM ammonium acetate and 0.1% formic acid in water and (B) 100% acetonitrile. The gradient conditions were 0 – 0.3 min, 2% B; 0.3–7.27 min, 2–80% B; 7.27–7.37 min, 80–98% B; 7.37–9.0 min, 98% B; 9–10 min 98–2% B; 10.0 – 15.0 min, 2% B at the flow rate of 0.5 mL/min. The ion source in the MS/MS system was electrospray ionization (EI) operated in either positive or negative ion mode with a capillary voltage of 1.5 kV. The ionization sources were programmed at 150°C and the desolvation temperature was programmed at 450°C. The optimized collision energy, cone voltage, molecular and product ions of biomarkers are summarized in Table 3.

Hsu S.Y., Bayati M.B., Li C., Hsieh H.Y., Belenchia A., Klutts J., Zemmer S.A., Reynolds M., Semkiw E., Johnson H.Y., Foley T., Wieberg C.G., Wenzel J., Johnson M.C, & Lin C.H. (2022). Biomarkers Selection for Population Normalization in SARS-CoV-2 Wastewater-based Epidemiology. medRxiv.

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