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Vnmrs console

Manufactured by Agilent Technologies
Sourced in United Kingdom, United States

The VNMRS console is a core component of Agilent's nuclear magnetic resonance (NMR) spectrometer system. It serves as the central control and processing unit, responsible for operating the NMR hardware and managing data acquisition, processing, and analysis. The VNMRS console provides the necessary functionality to perform various NMR experiments and generate high-quality, reliable data for scientific research and analytical applications.

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3 protocols using vnmrs console

1

In Vivo 13C MRS of Kidney Metabolism

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Data were acquired in a 9.4 T horizontal-bore magnet (Magnex Scientific, Abingdon, United Kingdom) with a VNMRS console (Varian, Palo Alto, CA, United States) and VnmrJ 3.2 (Agilent Technologies, Santa Clara, CA, United States). A surface coil equipped with a single loop tuned to 1H (400.2 MHz) and two 16 mm loops in quadrature tuned to 13C (100.67 MHz) was placed over the left kidney (to avoid signals from the liver), its position verified by 1H GEMS MRI, and shimmed using FASTESTMAP (Gruetter and Tkác, 2000 (link)). A series of 13C magnetic resonance spectra were acquired starting with the pyruvate infusion (∼2.9 s repetition time, with respiratory gating and cardiac triggering, 30° BIR4 adiabatic excitation, 20161.3 Hz spectral width, 8258 complex points, WALTZ-16 1H decoupling).
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2

MRI Measurements in Rodent Models

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In vitro and in vivo measurements were performed on a 9.4 T/ 31 cm actively shielded animal scanner (Magnex Scientific, Oxford, UK) equipped with a 12-cm-inner-diameter gradient (400 mT/m in x,y,z directions; Magnex Scientific) and interfaced to a VNMRS® console (Varian Inc., Palo Alto CA, USA). A custom-designed quadrature 1H surface coil consisting of two geometrically decoupled 16-mm diameter single loops, was used as transmitter/receiver probe. Bo field inhomogeneity was corrected using the FASTMAP protocol38 (link). In vitro tests were carried out on tap water phantoms. In vivo measurements were performed on male Sprague-Dawley rats with 350 gr average weights; the animals were anesthetized using 1.5% isoflurane and their physiology was monitored throughout the scans. All experiments were approved by the local ethics committee.
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3

Solid-state 13C CPMAS NMR Analysis

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Solid-state 13C CPMAS NMR [43 ] experiments were carried out in an 89-mm-bore 11.7T magnet using either an Agilent triple-resonance BioMAS probe with a DD2 console (Agilent Technologies) or a home-built four-frequency 1H/19F/13C/15N all-transmission-line probe with a Varian VNMRS console. Samples were spun at 7143 Hz in either 36-μl-capacity 3.2-mm zirconia rotors or thick-walled 5-mm-outer-diameter zirconia rotors. The field strength for cross-polarization was 50 kHz for 13C with ramped CP at 57 kHz on 1H for 1.5 ms. 1H decoupling was performed at 83 kHz. The recycle time was 2 s for all experiments. Spectrometer 13C chemical shift referencing was performed by setting the high-frequency adamantane peak to 38.5 ppm [44 (link)]. All spectra, except those obtained for the CP array experiments (Fig. S3 and S4), were the result of 40,960 scans. CP array experiments were performed at 0.25, 0.5, 1, 1.5, 2, 3, 4, 5, 6 ms. 8192 scans were collected for the CP array experiment of M. smegmatis mAGP. 16,384 scans were collected for the CP array experiment of M. abscessus mAGP.
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