SACC-LM and SACC-83 were obtained from Peking University and cultured in Roswell Park Memorial Institute-1640 (RPMI-1640) medium supplemented with 10% fetal bovine serum (FBS) at 37 ℃ in a humid incubator (Thermo Fisher Scientific, USA) containing 5% carbon dioxide (CO2). The 5-Azacytidine (5-Aza) and Cell Counting Kit-8 (CCK-8 kit) were obtained from APExBIO (Houston, USA); puromycin and cell lysis buffer for western blot (WB) and IP (immunoprecipitation) were purchased from Beyotime (Shanghai, China); and the Protein Quantification Kit (BCA kit) was purchased from Thermo Fisher.
Cell counting kit 8 cck 8 kit
Manufactured by Apexbio
Sourced in United States
The Cell Counting Kit-8 (CCK-8) is a colorimetric assay used for the determination of cell viability and cytotoxicity. It measures the number of viable cells in a sample by quantifying the amount of formazan dye produced by metabolically active cells. The CCK-8 kit is a simple and sensitive tool for cell proliferation and cytotoxicity studies.
Automatically generated - may contain errors
Lab products found in correlation
Puromycin, by Beyotime (1 mentions)
Cell lysis buffer for western blot, by Beyotime (1 mentions)
Humid incubator, by Thermo Fisher Scientific (1 mentions)
Lucifer yellow, by Merck Group (1 mentions)
Reactive oxygen species kit, by Beyotime (1 mentions)
Lactate dehydrogenase ldh, by Beyotime (1 mentions)
2 protocols using cell counting kit 8 cck 8 kit
1
Cell Culture Protocol for SACC-LM and SACC-83 Lines
2
In Vitro Evaluation of Tight Junction Integrity
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AA was purchased from Aladdin Chemical Co., Ltd. (Shanghai, China). OTA was purchased from Pribolab Biological Engineering Co., Ltd. (Qingdao, China). The Cell Counting Kit-8 (CCK-8) kit was bought from APExBIO Technology (Houston, TX, USA). Lactate dehydrogenase (LDH), Bicinconic Acid Assay (BCA), and reactive oxygen species (ROS) kits were obtained from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). Lucifer yellow (LY) was purchased from Sigma-Aldrich (St. Louis, MO, USA). ELISA kits were sourced from Boster Company (Wuhan, China). Antibodies against TJ (occludin, claudin-1, and ZO-1) and corresponding secondary antibodies were purchased from Abcam (Shanghai, China). All other chemical reagents in this research were of analytical grade.
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