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Daniovision recording chamber

Manufactured by Noldus

The Daniovision® recording chamber is a specialized piece of lab equipment designed for the observation and recording of zebrafish behavior. It provides a controlled environment for the study of zebrafish movements and interactions.

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3 protocols using daniovision recording chamber

1

Larval Locomotor Activity Monitoring

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Larvae (6 dpf) were separated into single wells of a 96-well plate containing 200 µL of E3 media and habituated in the Daniovision® recording chamber (Noldus) for 1 h before start of experiment. Larval locomotor activity was monitored over light–dark cycles using the Daniovision® apparatus. Analysis was performed using the Ethovision XT12 software (Noldus) to quantify the total swimming distance in given hours and the locomotor activity per second.
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2

Larval Locomotion and Touched-evoked Response

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At 7 days post-fertilization (dpf), larvae were transferred individually into a 96-well plate and activity was recorded over 4h/4h light-dark cycles using Basler GenIcam camera and DanioVision recording chamber (Noldus). Analysis was performed using the Ethovision XT 12 software (Noldus) to quantify the distance swam. For slow-motion imaging of touched-evoked response, glra1+/+ or -/- larvae were placed in a 10mm petri dish and the touch-evoked response was recorded at 120 fps using an iPhone 6 mounted on a iDu Optics cast from LabCam. Raw values are available in the Supporting Information (S1 Table).
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3

Larval Locomotor Activity Assay

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Larvae (5 dpf) were separated into single wells of a 96‐well plate containing 200 µl of E3 media and habituated in the Daniovision® recording chamber (Noldus) for 1 h before start of experiment. Larval locomotor activity was monitored over light‐dark cycles using the Daniovision® apparatus. Analysis was performed using the EthoVision XT12 software (Noldus) to quantify the total swimming distance in given hours and the locomotor activity per second.
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