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Sc 515387

Manufactured by Santa Cruz Biotechnology
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Sourced in United Kingdom

Sc‐515387 is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is designed for general scientific research applications. The core function of this product is to provide a specific technical capability, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab243634, by Abcam (2 mentions) Ab2811, by Abcam (2 mentions) Ab125243, by Abcam (1 mentions) Gst tag, by Cell Signaling Technology (1 mentions) His tag, by Cell Signaling Technology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Merck Group (1 mentions) Anti myc 2276, by Cell Signaling Technology (1 mentions) Actinomycin d, by Merck Group (1 mentions) Anti lamin a c, by Cell Signaling Technology (1 mentions) Anti p65 sc 8008, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Roche (1 mentions) Ab150081, by Abcam (1 mentions) Ab150160, by Abcam (1 mentions)

3 protocols using sc 515387

1

Co-immunoprecipitation of Protein Complexes

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Co‐immunoprecipitation was undertaken as documented,46, 47, 48, 49, 52, 53 using antibodies against CNBP (sc‐515387, Santa Cruz Biotechnology, Inc.), KPNB1 (ab2811), SMARCC2 (ab243634), Flag‐tag (ab125243, Abcam Inc.), Myc‐tag (#2276), His‐tag (#9991) or GST‐tag (#2624, Cell Signaling Technology). After being released from the bead‐bound complex, proteins were determined via western blotting or mass spectrometric assays (Wuhan SpecAlly Life Technology Co., Ltd., China).46, 47, 52, 53
Hu A., Chen G., Bao B., Guo Y., Li D., Wang X., Wang J., Li Q., Zhou Y., Gao H., Song J., Du X., Zheng L, & Tong Q. (2023). Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity. Clinical and Translational Medicine, 13(4), e1235.
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2

Characterization of CNBP and NF-κB Activation

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LPS (Escherichia coli 026:B6) and 1826 CpG-DNA (5΄-TsCsCsAsTsgsAsCsgsTsTsCsCsTsgsAsCsgsTsT-3΄) were purchased from Sigma-Aldrich (St Louis, MO, USA) and TIB Molbiol (Berlin, Germany), respectively. DAPI (4΄,6-diamidino-2-phenylindole) and actinomycin D were purchased from Sigma-Aldrich. The following antibodies were used: anti-CNBP (ab83038; Abcam, Cambridge, UK or sc-515387; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p65 (sc-8008; Santa Cruz Biotechnology), anti-tubulin (G094; ABM Inc., Richmond, Canada), anti-FLAG (G191; ABM Inc. and M185-3L; MBL International, Woburn, MA, USA), anti-HA (G036; ABM Inc.), anti-Myc (2276; Cell Signaling Technology), anti-GFP (11814460001; Roche, Basel, Switzerland and ab290; Abcam) and anti-Lamin A/C (2032; Cell Signaling Technology).
Lee E., Lee T.A., Kim J.H., Park A., Ra E.A., Kang S., Choi H.J., Choi J.L., Huh H.D., Lee J.E., Lee S, & Park B. (2017). CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6. Nucleic Acids Research, 45(6), 3280-3296.
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3

Immunofluorescence Staining of Cellular Proteins

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Cells were cultured on coverslips, which were treated by 10% goat serum for an hour at 37°C. Then, cells were treated by antibodies against KPNB1 (ab2811, Abcam Inc., dilution: 1:100), CNBP (sc‐515387, Santa Cruz Biotechnology, Inc., dilution: 1:100) or SMARCC2 (ab243634, Abcam Inc., dilution: 1:100) for 2 h. Coverslips were stained by 4′,6‐diamidino‐2‐phenylindole (300 nmol L−1) after incubating with Alexa Fluor 488 (ab150081) or Alexa Fluor 594 (ab150160, Abcam Inc.) goat anti‐rabbit IgG.
Hu A., Chen G., Bao B., Guo Y., Li D., Wang X., Wang J., Li Q., Zhou Y., Gao H., Song J., Du X., Zheng L, & Tong Q. (2023). Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity. Clinical and Translational Medicine, 13(4), e1235.
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