Lps tlrl eklps

Manufactured by InvivoGen

LPS (tlrl-eklps) is a laboratory reagent produced by InvivoGen. It is a purified lipopolysaccharide derived from Escherichia coli O111:B4.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Disuccinimidyl suberate, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 glutamax, by Thermo Fisher Scientific (1 mentions) Hoechst b2261, by Merck Group (1 mentions) Zvad fmk, by Bachem (1 mentions) Protein a sepharose beads, by GE Healthcare (1 mentions) Normal rabbit igg sc 2027, by Santa Cruz Biotechnology (1 mentions) Rabbit anti sqstm1, by Merck Group (1 mentions) Anti mouse 115 035 146, by Jackson ImmunoResearch (1 mentions)

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Tlrl-eklps (9 citations)

2 protocols using lps tlrl eklps

Cell Culture and Reagent Preparation for Immune Assays

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U937 (male) cells were cultured in RPMI 1640 Glutamax (Gibco) supplemented with 10% fetal calf serum (FCS; Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C and 5% CO₂. Human embryonic kidney (HEK) 293T (female) cells were cultured in Dulbecco’s modified Eagle’s medium Glutamax (Gibco) supplemented with 10% FCS (Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C and 5% CO₂. Cells were regularly tested for mycoplasma by polymerase chain reaction (PCR) analysis of culture supernatants (GATC Biotech). PMA (524400), nigericin (N7143), propidium iodide (PI) (P4170), KunB31 (SML2273), GM (SML1278), and Hoechst (B2261) were purchased from Sigma-Aldrich. Cycloheximide (A0879) was purchased from AxonLab. Disuccinimidyl suberate (A39267) was purchased from Thermo Fisher Scientific. Z-vad-fmk (4026865) was purchased from Bachem. Alum (tlrl-aloh) and LPS (tlrl-eklps) were purchased from InvivoGen. Doxycycline (A2951), ampicillin (A0839), and kanamycin (A1493) were purchased from AppliChem. E. cloni (60107) and ENDURA (60240) chemically competent bacteria were purchased from Lucigen.

Spel L., Hou C., Theodoropoulou K., Zaffalon L., Wang Z., Bertoni A., Volpi S., Hofer M., Gattorno M, & Martinon F. (2024). HSP90β controls NLRP3 autoactivation. Science Advances, 10(9), eadj6289.

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Immunoblot Analysis of Signaling Proteins

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Poly-L-lysine (P4832), dexamethasone/DEX (D4902-25MG), rabbit anti-ACTA1 (A2066) and rabbit anti-SQSTM1 (P0067) were purchased from Sigma. CpdA was synthesized according to the protocol described previously [49]. Chloroquine (sc-205,629), rabbit anti-NR3C1 (sc-8992x), mouse anti-LC3A/B (sc-398,822), rabbit anti-NFE2L2 (sc-13,032), rabbit anti-NFKBIA (C-21) and normal rabbit IgG (sc-2027) were from Santa Cruz Biotechnology. Horseradish peroxidase (HRP)-coupled secondary anti-rabbit (111–035-144) and anti-mouse (115–035-146) antibodies were purchased from Jackson ImmunoResearch. Rabbit anti-JUNB (C37F9), rabbit anti-ATG5 (2630), rabbit anti-p-MAPK/ERK (9101), mouse anti-MAPK/ERK (L34F12), mouse anti-p-MAPK/p38 (L28B10) and rabbit anti-MAPK/p38 (9212) were purchased from Cell Signalling Technology. Rabbit anti-HMOX1 (SPA-896) antibodies were from StressGen. Mouse anti-ubiquitin (VU-0101) antibodies were purchased from LifeSensors. Protein A Sepharose beads (17–5138-01) were from GE Healthcare Life Sciences. RNAse A (740,505) was from Macherey-Nagel. Proteinase K (19,131) was from Qiagen. LPS (tlrl-eklps) was purchased from Invivogen.

Mylka V., Deckers J., Ratman D., De Cauwer L., Thommis J., De Rycke R., Impens F., Libert C., Tavernier J., Vanden Berghe W., Gevaert K, & De Bosscher K. (2018). The autophagy receptor SQSTM1/p62 mediates anti-inflammatory actions of the selective NR3C1/glucocorticoid receptor modulator compound A (CpdA) in macrophages. Autophagy, 14(12), 2049-2064.

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