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Fam conjugated gene specific primers

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FAM conjugated gene specific primers are oligonucleotides designed to target and amplify specific gene sequences. The FAM (fluorescein amidite) fluorescent label is attached to the primers, enabling real-time detection and quantification of the target gene during PCR amplification.

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Lab products found in correlation

Quick rna miniprep kit, by Zymo Research (4 mentions) Taqman gene expression master mix, by Thermo Fisher Scientific (4 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (4 mentions) Mirvana isolation kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Specific primers (81 citations) Gene-specific primers (57 citations)

4 protocols using fam conjugated gene specific primers

1

Comprehensive Cardiac Gene Expression Analysis

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Total RNA was extracted using a Quick-RNA MiniPrep Kit according to the manufacturer’s instructions (Zymo Research). Total RNA (50–100 ng) was converted to complementary DNA (cDNA) using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). cDNA was used in a standard qPCR involving FAM-conjugated gene-specific primers (Thermo Fisher Scientific) and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific). Primers were acquired from Thermo Fisher Scientific, and the following are the assay ID numbers: Actn2 Mm00473657_m1; Cacna1c Mm00437917_m1; Gapdh Mm99999915_m1; Kcna4 Mm01336166_m1; Kcnh2 Mm00465377_mH; Kcnj2 Mm00434616_m1; Kcnk6 Mm01176312_g1; Myh6 Mm00440359_m1; Myoz2 Mm00469639_m1; Nebl Mm00503886_m1; Ryr2 Mm00465877_m1; Scn5a Mm01342518_m1; Tnnc1 Mm00437111_m1; Tnni1 Mm00502426_m1; Tnni3 Mm00437164_m1; Tnnt2 Mm01290256_m1; and Ttn Mm00621005_m1.
Baksh S.S., Pratt R.E., Gomez J., Dzau V.J, & Hodgkinson C.P. (2022). A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes. The Journal of Biological Chemistry, 298(6), 102053.
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2

qPCR Gene Expression Analysis Protocol

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using Quick-RNA MiniPrep Kit according to the manufacturer’s instructions (Zymo Research). Total RNA (50 ng–100 ng) was converted to cDNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems). cDNA was used in a standard qPCR reaction involving FAM conjugated gene-specific primers (ThermoFisher) and TaqMan Gene Expression Master Mix (ThermoFisher). Primers were acquired from ThermoFisher (2 (link), 5 (link), 6 (link)).
Sun H., Pratt R.E., Dzau V.J, & Hodgkinson C.P. (2023). Neonatal and adult cardiac fibroblasts exhibit inherent differences in cardiac regenerative capacity. The Journal of Biological Chemistry, 299(5), 104694.
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3

Quantifying Cardiac miRNAs and mRNAs

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miRNA: miRNAs were isolated with a MirVana Isolation kit (ThermoFisher) according to the manufacturer’s protocol. Expression of the miRNAs was determined in a standard qPCR reaction involving FAM conjugated specific primers (ThermoFisher) and TaqMan Gene Expression Master Mix. Briefly, miR combo expression levels were measured by absolute quantification using the Standard Curve Method. Reverse transcription products (cDNA) from miR precursor mimics to miR-1, miR-133, miR-208, and miR-499 (Ambion) were serially diluted and qPCR was performed to generate standard curves to correct for differences in primer efficiencies. Assay ID numbers for the primers employed: miR-1 477820_mir; miR-133 rno480920_mir; miR-208 477819_mir; miR-499 rno481402_mir. RNA: Total RNA was extracted using Quick-RNA MiniPrep Kit according to the manufacturer’s instructions (Zymo Research). Total RNA (50ng-100ng) was converted to cDNA using a high capacity cDNA reverse transcription kit (Applied Biosystems). cDNA was used in a standard qPCR reaction involving FAM conjugated gene specific primers (ThermoFisher) and TaqMan Gene Expression Master Mix (ThermoFisher). Primers were acquired from ThermoFisher and the assay ID numbers are: Mef2C Mm01340842_m1; Myh6 Mm00440359_m1.
Kang M.H., Hu J., Pratt R.E., Hodgkinson C.P., Asokan A, & Dzau V.J. (2020). Optimizing Delivery for Efficient Cardiac Reprogramming. Biochemical and biophysical research communications, 533(1), 9-16.
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4

Comprehensive Cardiac Gene Expression Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using Quick-RNA MiniPrep Kit according to the manufacturer’s instructions (Zymo Research). Total RNA (50ng-100ng) was converted to cDNA using a high capacity cDNA reverse transcription kit (Applied Biosystems). cDNA was used in a standard qPCR reaction involving FAM conjugated gene specific primers (ThermoFisher) and TaqMan Gene Expression Master Mix (ThermoFisher). Primers were acquired from ThermoFisher and the assay ID numbers are: Actn2 Mm00473657_m1; Cacna1c Mm00437917_m1; Gapdh Mm99999915_m1; Kcna4 Mm01336166_m1; Kcnh2 Mm00465377_mH; Kcnj2 Mm00434616_m1; Myh6 Mm00440359_m1; Myoz2 Mm00469639_m1; Neb Mm01546298_m1; Nebl Mm00503886_m1; Ryr1 Mm01175211_m1; Ryr2 Mm00465877_m1; Scn4a Mm00500103_m1; Scn5a Mm01342518_m1; Tnnc1 Mm00437111_m1; Tnni1 Mm00502426_m1; Tnni2 Mm00437157_g1; Tnni3 Mm00437164_m1; Tnnt2 Mm01290256_m1; Ttn Mm00621005_m13 (link).
Harris S., Anwar I., Baksh S.S., Pratt R.E., Dzau V.J, & Hodgkinson C.P. (2024). Skeletal muscle differentiation induces wide-ranging nucleosome repositioning in muscle gene promoters. Scientific Reports, 14, 9396.
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