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Ck636

Manufactured by Merck Group
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Sourced in Israel, Sao Tome and Principe

The CK636 is a versatile laboratory centrifuge designed for a wide range of applications. It features a robust and compact design, enabling efficient separation of a variety of sample types. The CK636 is capable of reaching high speeds, ensuring effective separation and sedimentation of sample components.

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Lab products found in correlation

Fibronectin, by Thermo Fisher Scientific (1 mentions) Cytob, by Merck Group (1 mentions) Y 27632, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Blebbistatin, by Merck Group (1 mentions) β mercaptoethanol, by Merck Group (1 mentions) Do11.10 t cell receptor transgenic mice, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Ck548, by Merck Group (1 mentions) Alexa fluor 633 conjugated goat anti rat igg, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by GenScript (1 mentions)

4 protocols using ck636

1

Cytoskeleton Modulation Reagents

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Fibronectin (PHE0023) was purchased from Gibco. Blebbistatin (D00155970) and Dynamin Inhibitor Dynasore (D00098387) were purchased from Calbiochem. CytoB (C6762), CK636 (C7374), and Latruculin A (L5163) were from Sigma-Aldrich.
Ma L., Li Y., Peng J., Wu D., Zhao X., Cui Y., Chen L., Yan X., Du Y, & Yu L. (2014). Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Research, 25(1), 24-38.
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2

Investigating Actin Dynamics in Fibrin

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To examine the effect of ARP2/3 inhibition on band formation, fibrin gels were treated with CK636 (Sigma-Aldrich, Israel). Gels were polymerized as described above and the medium was replaced with DMEM containing 100 µM CK636, immediately after gel polymerization.
Natan S., Koren Y., Shelah O., Goren S, & Lesman A. (2020). Long-range mechanical coupling of cells in 3D fibrin gels. Molecular Biology of the Cell, 31(14), 1474-1485.
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3

Generating DO11.10 T Cell Blasts

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DO11.10 T cell receptor transgenic mice (Jackson Laboratories) bred in the POSTECH Biotech Center (PBC) were used to generate DO11.10 T cell blasts. All the T cell assay including mice treatments were performed in accordance with the guidelines approved by the Institutional Animal Care and Use Committee at PBC. DO11.10 blasts were obtained by stimulating cells in the spleen and lymph nodes of DO11.10 transgenic mice with 1 μg/ml of OVA323–339 peptide (ISQAVHAAHAEINEAGR, Peptron, Inc. Korea). Cells were cultured in RPMI 1640 (Invitrogen) containing 10% of FBS, 1% of penicillin-streptomycin and 0.1% of beta-mercaptoethanol (Sigma). 1–2 U/ml of IL-2 was added on the 2nd day of stimulation and cells were used for experiments on the 5th days. To inhibit arp2/3 complex, myosin light chain kinase (MLCK), ROCK and non-muscle myosin II, CK636 (100 μM, Sigma), ML7 (30 μM, Sigma), Y-27632 (50 μM, Sigma) and blebbistatin (50 μM, Sigma) were incubated with cells for 1 h, respectively. For arp2/3 complex inhibition, CK666, which is a more potent derivative of CK63635 (link), can be used, instead.
Song K.H., Lee J., Jung H.R., Park H, & Doh J. (2017). Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation. Scientific Reports, 7, 11533.
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4

Antibody Characterization for Cell Signaling

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The rat mAb M1/70, which recognizes the mouse αM integrin subunit, was purified from the conditioned media of hybridoma cells (obtained from The American Tissue Culture Collection, Manassas, VA) using protein A agarose. The mouse anti-talin (T3287) and anti-vinculin (V9131) mAbs and rabbit anti-I/S-afadin polyclonal antibody (A0224) were from Sigma (St. Louis, MO).
The rabbit anti-paxillin (32084) and anti-JAM-A (180821) polyclonal antibodies and rabbit anticortactin mAb conjugated to Alexa Fluor 555 were from Abcam (Cambridge, MA). The rat antinectin-2 (502-57) mAb was from Santa Cruz Biotechnology (Dallas, TX). The mouse anti-Ecadherin mAb (24E10) and rabbit anti-Myosin IIa polyclonal antibody (34035) were from Cell Signaling (Danvers, MA). The rat anti-SIRPα/CD172a polyclonal antibody (P84; 552371) was from BD Bioscience (San Jose, CA). The rabbit anti-MT1-MMP-14 polyclonal antibody (14552-1-AP) was from Proteintech (Rosemont, IL). The rabbit anti-ZO-1 polyclonal antibody (61-7300) and secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 633conjugated goat anti-rat IgG were from Invitrogen (Carlsbad, CA). Vibrant DiD membranelabeling reagent was from Thermo Fisher (Waltham, MA). Brewer's thioglycollate (TG), wiskostatin, and the Arp2/3 inhibitors CK-548 and CK-636 were from Sigma (St. Louis, MO). IL-4 was from Genscript (Piscataway, NJ).
Balabiyev A., Podolnikova N.P., Mursalimov A., Lowry D., Newbern J.M., Roberson R.W., & Ugarova T.P. (2020). TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLS.
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