Immunohistochemistry was performed on colon tumors from the AOM model as previously described (20 (link)). Briefly, the fixed sections cut from frozen blocks were blocked with 10% goat serum (Vector Labs) for 1 hour at room temperature then incubated overnight with rat-anti-mouse CD8 (clone KT15; 1:100; AbD Serotec) or rat-anti-mouse CD4 (clone 4SM95; 5 µg/ml; eBioscience). After extensive washing, the slides were incubated with Alexa Fluor 488 goat-anti-rat (Abcam; 1:500) for 1 hour at room temperature. Cover slips were mounted with Prolong Gold antifade with DAPI (Life technologies). Positive cells and DAPI stained nuclei were counted in three random high-powered fields per slide and expressed as a mean percent of positive stained cells of total cell counted.
Rat anti mouse cd8 clone kt15
Manufactured by Bio-Rad
The Rat-anti-mouse CD8 (clone KT15) antibody is a laboratory reagent used to detect the presence of CD8 marker on mouse cells. It is a rat monoclonal antibody that binds specifically to the CD8 antigen expressed on the surface of certain T cells and other immune cells in mice.
Automatically generated - may contain errors
2 protocols using rat anti mouse cd8 clone kt15
1
Immunohistochemistry Analysis of Colon Tumors
2
Quantifying Tumor-Infiltrating CD8+ T-Cells
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