Roseburia intestinalis L1-82 (DSMZ14610) was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany). Bacteroides fragilis (ATCC 25285) and Bacteroides vulgatus were obtained from Ningbo Mingzhou Biotechnology Co., Ltd. (Zhejiang, China). The above bacteria were cultured under anaerobic conditions at 37°C. An anaerobic culture environment was created using Atmosphere Generation Systems (AnaeroJar™ ASSEMBLY and AnaeroGen™, OXOID, Thermo Fisher Scientific). The culture medium (Miquel et al., 2015 (link)) was brain heart infusion (BHI) containing 0.5% yeast extract (OXOID), 1 mg/ml cellobiose (Macklin, China), 1 mg/ml maltose (Solarbio, China), 0.5 mg/ml cellobiose (Macklin, China), and 0.5 mg/ml cysteine (Solarbio, China). We established a standard curve to convert the absorbance values at 600 nm (OD600) to CFU values (Supplementary Figure S1 ). Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Salmonella typhimurium CMCC 50115, Bacillus subtilis DSM 1088, and Bacillus cereus ATCC 11778 were used as controls. These strains were cultured in nutrient broth at 37°C in an aerobic environment.
Maltose
Manufactured by Solarbio
Sourced in China
Maltose is a disaccharide sugar composed of two glucose molecules linked together. It is a commonly used laboratory reagent that serves as a carbon source and energy substrate for microbial growth and fermentation processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using maltose
1
Anaerobic Gut Bacteria Cultivation Protocol
2
Recombinant CsAGA2 Protein Purification
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The full-length CsAGA2 CDS was cloned into pMAL-C2X vector (to create MBP fusion protein) using homologous recombination, and the recombinant plasmid was then transformed into Escherichia coli BL21 for heterologous protein expression. When the culture reached an optical density 600 (OD 600 ) of 0.6-0.8, 0.2-mM isopropyl-β-d-thiogalactoside (IPTG) was added and shaken at 16°C for 16 h. The culture was centrifuged at 5,000 rpm for 8 min at 4°C, and then the cells were resuspended in 10 mL MBP column buffer ), 1-mM PMSF]. The transformed cells were disrupted via sonication on ice for 15 min. The recombinant protein was purified using 500-µL MBP buffer containing magnetic beads (NEB, Beijing, China), and eluted with 75-µL MBP buffer containing 10-mM maltose (Solarbio, Beijing, China) .
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