DNAs were purchased from
Eurofins Genomics
(Tokyo, Japan). Nonmodified and FAM-modified DNAs were of oligonucleotide
purification cartridge grade, and Alexa 405- and Cy3-modified DNAs
were of high-performance liquid chromatography purification grade.
DNA was stored in Ultrapure water (18 MΩ·cm in resistance)
at a concentration of 100 μM at −20 °C until use.
DNA concentration was measured using a microvolume spectrometer (DS-11FX,
DeNovix, Wilmington, DE, USA). DNA sequences are provided inTable S1 . Sorbitan monooleate (Span 80) and oleylamine
were purchased from Tokyo Chemical Industry (Tokyo, Japan) and Kanto
Chemical (Tokyo, Japan), respectively. Ultrapure water and Tris-HCl
(pH 8.0) were purchased from Thermo Fisher Scientific (Waltham, MA,
USA). NaCl powder, cholesterol, polyoxyethylene (10) octylphenyl ether
(Triton-X 100), sucrose, and liquid paraffin were purchased from FUJIFILM
Wako Pure Chemical (Osaka, Japan). Iodixanol (OptiPrep) was purchased
from Cosmo Bio (Tokyo, Japan). Silicone-coated glasses (30 mm by 40
mm with a thickness of 0.17 mm) were purchased from Matsunami Glass
(Osaka, Japan). 1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC) was purchased from NOF Corporation (Tokyo, Japan). 1,2-Dioleoyl-3-trimethylammonium-propane
(DOTAP) was purchased from Avanti Polar Lipids (Alabaster, AL, USA).
Exonuclease I and III were purchased from New England Biolabs (Ipswich,
MS, USA).
Eurofins Genomics
(Tokyo, Japan). Nonmodified and FAM-modified DNAs were of oligonucleotide
purification cartridge grade, and Alexa 405- and Cy3-modified DNAs
were of high-performance liquid chromatography purification grade.
DNA was stored in Ultrapure water (18 MΩ·cm in resistance)
at a concentration of 100 μM at −20 °C until use.
DNA concentration was measured using a microvolume spectrometer (DS-11FX,
DeNovix, Wilmington, DE, USA). DNA sequences are provided in
were purchased from Tokyo Chemical Industry (Tokyo, Japan) and Kanto
Chemical (Tokyo, Japan), respectively. Ultrapure water and Tris-HCl
(pH 8.0) were purchased from Thermo Fisher Scientific (Waltham, MA,
USA). NaCl powder, cholesterol, polyoxyethylene (10) octylphenyl ether
(Triton-X 100), sucrose, and liquid paraffin were purchased from FUJIFILM
Wako Pure Chemical (Osaka, Japan). Iodixanol (OptiPrep) was purchased
from Cosmo Bio (Tokyo, Japan). Silicone-coated glasses (30 mm by 40
mm with a thickness of 0.17 mm) were purchased from Matsunami Glass
(Osaka, Japan). 1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC) was purchased from NOF Corporation (Tokyo, Japan). 1,2-Dioleoyl-3-trimethylammonium-propane
(DOTAP) was purchased from Avanti Polar Lipids (Alabaster, AL, USA).
Exonuclease I and III were purchased from New England Biolabs (Ipswich,
MS, USA).