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Anti yap1 antibody

Manufactured by Abnova

The Anti-YAP1 antibody is a laboratory reagent used to detect and study the expression of the YAP1 protein in biological samples. YAP1 is a transcriptional regulator that plays a key role in the Hippo signaling pathway, which is involved in the regulation of cell growth, proliferation, and organ size. The Anti-YAP1 antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to analyze the presence and localization of YAP1 in cells and tissues.

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2 protocols using anti yap1 antibody

1

Western Blot Analysis of Histone Deacetylases

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RPMI culture medium was purchased from Corning (Corning, NY). Fetal bovine serum (FBS) was purchased from Sigma Chemical Co. (St. Louis, MO). Trypsin, pen/strep antibiotics, and puromycin were purchased from Gibco (Grand Island, NY). Trametinib (MEK inhibitor) was purchased from Selleckchem (Houston, TX). Antibodies for Western Blot: Anti-HDAC1 (#2062, from Cell Signaling Technology (Danvers, MA), anti-HDAC2 (#2540 CST), The anti-HDAC3 and anti-HDAC8 antibodies were described in refs. 17 (link), HDAC11(#58442, CST). Anti-HDAC6 (#H-300, sc-11420) was purchased from Santa Cruz Biotechnologies. Cleaved PARP (Asp214) (#D64E10) (#5625 CST), phospho LKB1 (#3482, CST), total LKB1 (#3050, CST). Anti-YAP1 antibody (#H00010413-M01, Abnova). Anti-Vinculin (#G8796) and anti-GAPDH (#V9131) were purchased from Millipore Sigma (Bedford, MA).
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2

Western Blot Analysis of Histone Deacetylases

Check if the same lab product or an alternative is used in the 5 most similar protocols
RPMI culture medium was purchased from Corning (Corning, NY). Fetal bovine serum (FBS) was purchased from Sigma Chemical Co. (St. Louis, MO). Trypsin, pen/strep antibiotics, and puromycin were purchased from Gibco (Grand Island, NY). Trametinib (MEK inhibitor) was purchased from Selleckchem (Houston, TX). Antibodies for Western Blot: Anti-HDAC1 (#2062, from Cell Signaling Technology (Danvers, MA), anti-HDAC2 (#2540 CST), The anti-HDAC3 and anti-HDAC8 antibodies were described in refs. 17 (link), HDAC11(#58442, CST). Anti-HDAC6 (#H-300, sc-11420) was purchased from Santa Cruz Biotechnologies. Cleaved PARP (Asp214) (#D64E10) (#5625 CST), phospho LKB1 (#3482, CST), total LKB1 (#3050, CST). Anti-YAP1 antibody (#H00010413-M01, Abnova). Anti-Vinculin (#G8796) and anti-GAPDH (#V9131) were purchased from Millipore Sigma (Bedford, MA).
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