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4 protocols using tetrachlorauric acid

1

Synthesis of Gold Nanoparticles and Nitrophenol Reduction

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2-2′-Azo-bis-isobutyronitrile (AIBN), vinyl acetate (>99%), tetrachlorauric acid (HAuCl4·3H2O), sodium borohydride (NaBH4, 99%), activated carbon NORIT SX1G, sulfuric acid (H2SO4, 96%), 4-nitrophenol (>99%), 3-nitrophenol (>99%), 2-nitrophenol (>99%), polyvinyl alcohol 88 (Mw: 13,000–23,000, 88–89% hydrolyzed), polyvinyl alcohol 2 (Mw: 13,000–23,000, 98–99% hydrolyzed), polyvinyl alcohol 3 (Mw: 31,000–50,000, 98–99% hydrolyzed), and polyvinyl alcohol 4 (Mw: 146,000–186,000, 98–99% hydrolyzed) were purchased from Sigma Aldrich (Italy). The other polymers were properly synthesized in the laboratory and experimental protocols are presented below. The solvents were used without further processes of purification.
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2

Colorimetric Cytotoxicity Assay Protocol

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Tetrachlorauric acid (HAuCl4.3H2O), Trisodium Citrate salt, Dimethylsulphoxide (DMSO),RPMI-1640 medium,Sodium bicarbonate,Trypan blue, Fetal Bovine Serum, Penicillin/Streptomycin,Trypsin,Acetic acid, Sulphorhodamine-B (SRB),TrichloroAcetic acid (TCA), Tris base 10 mM (PH 10.5) are obtained from Sigma Aldrich Chemical Co., St.Louis, Mo, U.S.A.
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3

Biosynthesis of Nanoparticles from Waste

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The materials utilized for biosynthesis consisted of dried Vitis vinifera canes which were collected during dormancy period and waste material after Cannabis sativa cannabinoid extraction, both located from sources in the Czech Republic. Additional materials included silver nitrate and tetrachlorauric acid obtained from Sigma Aldrich, ethanol (40%), and distilled water, ultrapure water.
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4

Functionalized Gold Nanoparticles for MCF-7 Cytotoxicity

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All the glassware was washed with aqua regia [HCl:HNO3 = 3:1 (v/v)] and then rinsed with deionized water. Tetrachlorauric acid (HAuCl4·3H2O), 6-mercaptopurine, chitosan low molecular weight (mol wt 50,000–190,000 Da), dimethylsulphoxide (DMSO), RPMI-1640 medium, sodium bicarbonate, trypan blue, fetal Bovine Serum (FBS), trypsin, acetic acid, sulphorhodamine-B (SRB), trichloroacetic acid (TCA) and tris base 10 mM (PH 10.5) were obtained from Sigma Aldrich Chemical Co., St. Louis, Mo, USA.
The human breast carcinoma cell line (MCF7) was obtained from the American Type Culture Collection (ATCC, MO, USA). The tumor cell line was propagated and maintained by serial sub-culturing in RPMI-1640 medium containing 10% FBS and 1% penicillin/streptomycin.
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