1 n naoh

NIS-mediated uptake of ¹²⁵I in HSP70B-NIS- MSCs was measured as described previously 49 (link), 50 (link). Briefly, cells were seeded on 12-well plates and iodide uptake studies were performed at different time points (0 - 24 h) after heat treatment. Cells were incubated in Hanks' Balanced Salt solution (Gibco/Life Technologies, Carlsbad, California, USA), complemented with 10 µM NaI, 100 000 counts per minute (cpm) of Na¹²⁵I/ml (PerkinElmer, Waltham, Massachusetts, USA) and 10 mM 4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid (HEPES; Sigma) (pH 7.3) for 45 min. The NIS-specific inhibitor KClO₄ (100 mM; Merck Millipore, Burlington, Massachusetts, USA) was added to control wells to verify NIS specificity of uptake. After washing, cells were lysed in 1 N NaOH (Carl Roth GmbH + Co KG, Karlsruhe, Germany) for 15 min and trapped ¹²⁵I was analyzed by γ-counting (Beckman Coulter GmbH, Krefeld, Germany). Results were normalized to cell survival (see below) and expressed as cpm / A620.

The following wound dressings were selected for this study: alginate (Suprasorb^® A, Lohmann & Rauscher GmbH & Co. KG, Germany) and alginate + ionic-Ag (Suprasorb^® A + Ag, Lohmann & Rauscher GmbH & Co. KG, Germany), alginate + nano-Ag (Acticoat^◊ Absorbent, Smith & Nephew GmbH, Germany), sodium carboxymethylcellulose (CMC) (AQUACEL^®, ConvaTec GmbH, Germany) CMC with Ag⁺ (AQUACEL^® Ag, ConvaTec GmbH, Germany) as well as polyurethane (PU)-foam with TLC (UrgoCell^®, URGO GmbH, Germany) and PU-foam with TLC/Ag⁺ (UrgoCell^® silver, URGO GmbH, Germany). Active ingredients and wound dressing basic materials are specified in Table 2 according to the manufacturers’ descriptions.
Staphylococcus aureus ATCC 6538 and P. aeruginosa ATCC 27853 were obtained from the DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen, Germany). For cultivation of bacteria, special peptone and “lab-lemco” powder for preparation of caso-bouillon and bacteriological agar were purchased from Oxoid (UK). Columbia agar plates with 5 % sheep blood and MH2 agar plates were acquired from Biomeriéux (France).
0.9 % NaCl solution was purchased from Fresenius Kabi Deutschland GmbH (Germany). 1 N HCl, 1 N NaOH, and Tween 20 were obtained from Carl Roth GmbH (Germany).