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Dl isocitric acid trisodium salt hydrate

Manufactured by MP Biomedicals
Sourced in United States

Dl-isocitric acid trisodium salt hydrate is a chemical compound used in various laboratory applications. It serves as a source of isocitric acid, a key intermediate in the citric acid cycle. The compound is available in a hydrated form.

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2 protocols using dl isocitric acid trisodium salt hydrate

1

Isocitrate Dehydrogenase Enzyme Assay

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Tris–hydrochloride, sodium chloride, magnesium chloride hexahydrate, αKG (sodium salt), and resazurin were purchased from Fisher (Hampton, NH). The pH of αKG stocks was adjusted to 7.0 before use. dl-isocitric acid trisodium salt hydrate was purchased from MP Biomedicals (Santa Ana, CA). NADPH (tetrasodium salt) and NADP+ (disodium salt) were purchased from Calbiochem (San Diego, CA). Diaphorase from Clostridium kluyveri and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO). Bovine serum albumin (BSA) was purchased from SeraCare Lifescience (Milford, MA). ML309 was purchased from Sigma, but the manufacturer unable to confirm if the compound is stereospecific or racemic (Sigma–Aldrich, St. Louis, MO). AGI-5198 was purchased from Selleckchem (Houston, TX). GSK864 was purchased from Cayman Chemical Company (Ann Arbor, MI).
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2

Assay for α-Ketoglutarate Metabolism

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Tris–hydrochloride, sodium chloride, magnesium chloride hexahydrate, and α-ketoglutarate (αKG, sodium salt) were purchased from Fisher (USA). The pH of αKG stocks was adjusted to 7.0 before use. DL-isocitric acid trisodium salt hydrate was purchased from MP Biomedicals (USA). NADPH (tetrasodium salt) and NADP+ (disodium salt) were purchased from Calbiochem (USA). Dimethyl sulfoxide (DMSO) was purchased from Sigma–Aldrich (USA). Bovine serum albumin (BSA) was purchased from SeraCare Lifescience (USA).
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