Ack rbc lysis buffer
ACK RBC lysis buffer is a solution used to lyse (break down) red blood cells (RBCs) in samples. It effectively removes RBCs from cell suspensions, allowing for the isolation and analysis of other cell types.
Lab products found in correlation
6 protocols using ack rbc lysis buffer
Immunophenotyping of Blood Leukocytes
Lung Tissue Dissociation Protocol
Isolation and Immunophenotyping of Splenocytes
Medulloblastoma Tumor Tissue Dissociation
The tumor sample was mechanically dissociated in a solution containing Hank’s Balanced Salt Solution (HBSS), nonessential amino acids, sodium pyruvate, sodium bicarbonate, HEPES, Glutamax-1, antibiotic-antimycotic, DNase, and collagenase IV. All media components were from Cellgro, except for DNase and collagenase IV, which were from Worthington. The suspension was washed two times with HBSS, filtered through a 70-μm strainer and resuspended in a 0.9 M sucrose solution in HBSS without Ca2+/Mg2+ (Cellgro) to remove debris and dead cells. The cells were treated with ACK/RBC lysis buffer (Gibco), then washed twice in phosphate buffered saline (PBS). Cells were plated in tumor stem media (TSM) consisting of Neurobasal-A medium (Invitrogen), B27-A (Invitrogen), human bFGF (20 ng/ml) (Shenandoah Biotech), human EGF (20 ng/ml) (Shenandoah Biotech), human recombinant LIF (20 ng/ml) (Millipore), and heparin (10 ng/ml).
Medulloblastoma Tumor Tissue Dissociation
Spleen Cell Isolation and ELISPOT Assay
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