Hrp from amoracia rusticana

The ɛ-caprolactam (ECL) monomer with reduced moisture content for anionic polymerization (AP-Nylon^®) was purchased from Brüggemann Chemical (Heilbronn, Germany). Before use, it was kept under vacuum for 1 h at 23 °C. As a polymerization activator, Brüggolen C20^® (C20) from the same company was used. The initiator sodium dicaprolactamato-bis-(2-methoxy-ethoxo)-aluminate (Dilactamate^®, DL, 85% solution in Toluene) was purchased from Katchem (Prague, Czech Republic) and applied without further treatment. Toluene, xylene, methanol and other solvents were all of analytic grade, purchased from Merck (Lisbon, Portugal) and used as received. GOx from Aspergillus Niger type VIII, D–(+) glucose (purum p.a.), NaH₂PO₄·2H₂O, 99% (purum p.a.), Na₂HPO₄·2H₂O, (98%), NaCl (99%) and CH₃COOH (99%) were purchased from Merck/Sigma Aldrich, Lisbon, Portugal. HRP from Amoracia rusticana and CH₃COONa (99%) were purchased from Alfa Aesar (Lancashire, UK). 3,3′,5,5′-Tetramethylbenzidine (TMB, 99%) was purchased from Acros Organic (Porto Salvo, Portugal). Pebax MH 1657 resin (block copolymer with 60% soft polyethylene oxide segments and 40% PA6 hard segments) was kindly donated by Arkema (Serquigny, France), and poly(ethylene terephthalate) film (Melinex^®) was supplied by Addev Materials (Lyon, France).

The ɛ-caprolactam (ECL) monomer with reduced moisture content for anionic polymerization (AP-Nylon^®) was delivered from Brüggemann Chemical (Heilbronn, Germany). Before use, it was kept under vacuum for 1 h at 23 °C. A polymerization activator, Brüggolen C20^® (C20), from the same company, was used. The initiator sodium dicaprolactamato-bis-(2-methoxy-ethoxo)-aluminate (Dilactamate^®, DL, 85% solution in Toluene) was purchased from Katchem (Prague, Czech Republic) and applied without further treatment. Toluene, xylene, methanol, and other solvents were all of analytic grade, purchased from Merck (Lisbon, Portugal) and used as received. Gox from Aspergillus niger type VIII, D−(+) glucose (purum p.a.), NaH₂PO₄·2H₂O, 99% (purum p.a.), Na₂HPO₄·2H₂O, (98%), NaCl (99%), and CH₃COOH (99%) were purchased from Merck/Sigma Aldrich, Lisbon, Portugal. HRP from Amoracia rusticana and CH₃COONa (99%) were purchased from Alfa Aesar (Lancashire, UK). 3,3′,5,5′-tetramethylbenzidine (TMB, 99%) and CuSO₄.5H₂O (99%) were purchased from Acros Organic (Porto Salvo, Portugal).