Twenty microliters of the above mentioned Ligia extracts, PE, EE, ME-1, ME-2, ME-3, 95E, and 50E, were analyzed with an Agilent HPLC device coupled to an diode array detector and a C18 reversed phase column (Waters, Xbridge, 250 × 4.6 mm, 5 μm) was used. The HPLC profiling was performed at room temperature, and the flow rate was set at 1 mL/min. The mobile phases consisted of purified water (A) and methanol (B). The elution gradient was set as follows: 0–5 min, 5% B; 5–40 min, 5-100% B; 40–55 min, 100% B; 55–60 min, 100–5% B.
Hplc device
Manufactured by Agilent Technologies
Sourced in Germany, United States
The HPLC device is a laboratory instrument used for the separation, identification, and quantification of various chemical compounds. It operates by utilizing high pressure to pump a liquid mobile phase through a stationary phase, allowing for the separation of the sample components based on their interaction with the two phases.
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3 protocols using hplc device
1
HPLC Analysis of Ligia Extracts
2
Analysis of Fermentation Products
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The samples were clarified through centrifugation (13,000 ×g, 5 min, 4°C) and filtration (0.22 μm cellulose acetate filter) and stored at −20°C until analyzed. Fermentation products (formic, acetic, lactic, propionic, butyric, and succinic acids and ethanol) were analyzed using a HPLC device (Agilent technologies, Waldbronn, Germany) equipped with refractive index detector and Aminex HPX-87 H ion exclusion column. Isocratic elution was carried out with 0.005 M H2SO4 at 0.6 mL/min [33 (link)].
3
Melamine Quantification by HPLC
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HPLC device (Agilent Technologies, USA), ultrasonic instrument (Elma, Germany), micro-scale (µg) digital balance (Sartorius, Germany), sampler (Eppendorf, Germany), HPLC quality deionized water (multi-Q filter system), Melamine (Sigma, Germany, Lot number: 1422105v), Acetonitrile HPLC Grade (Merck, Germany), pure ethanol (Merck, Germany).
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