Peripheral blood mononuclear cells were stained with fluorochrome-conjugated mAbs for flow cytometric analysis. CD3/CD8/CD45/CD4 (Cat# 340499, RRID: AB_400472; BD Biosciences, San Jose, CA, USA) and anti-CD3/CD16+CD56/CD45/CD19 (BD Biosciences Cat# 340500, RRID:AB_400473) were used. Flow cytometry was performed using a FACSAria II (BD Biosciences), and the data were analyzed with FlowJo software (Tree Star, Ashland, OR, USA). CD4+ T lymphocytes were defined as CD45+CD3+CD4+CD8− cells. CD8+ T lymphocytes were defined as CD45+CD3+CD4−CD8+ cells. NK cells and B lymphocytes were defined as CD45+CD3−CD16+56+CD19− cells and CD45+CD3−CD16−56−CD19+ cells.
Cd3 cd8 cd45 cd4
Manufactured by BD
Sourced in United States
The CD3/CD8/CD45/CD4 lab equipment product is a tool used for the identification and enumeration of T-cell subsets, including helper T-cells (CD4+) and cytotoxic T-cells (CD8+), as well as the total T-cell population (CD3+). It utilizes flow cytometry technology to provide accurate and reliable measurements of these cell types in biological samples.
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Lab products found in correlation
2 protocols using cd3 cd8 cd45 cd4
1
Multiparametric Flow Cytometry Analysis
2
Multicolor Flow Cytometry of T-cell and B-cell Subsets
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Two sets of four-color florescent antibody, CD3/CD8/CD45/CD4 (BD Biosciences) and CD3/CD16+ CD56/CD45/CD19 (BD Biosciences), were added (20 μl each) into two separate flow cytometry tubes. The sample (50 μl whole blood each) was added to each tube, followed by incubation at room temperature (RT) under darkness for 15 min. After adding 0.45 ml erythrocytolysin (BD Biosciences), the solution was incubated for another 10 min. The solution was then centrifuged for 5 min at 1200 rpm. The supernatant was discarded and the pellet was washed twice with 2 ml PBS. After resuspension in 0.4 ml phosphate-buffered saline (PBS), the sample was loaded for flow cytometry analysis. Data analyses were performed with MultiSET software (BD Biosciences). Measurements included percentages of B cells (CD19+), T cells (CD3+), CD4+ T cells, CD8+ T cells and NK cells (CD16+CD56+) and the ratio of CD4+/CD8+ T cells.
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