Phospho mtor

Manufactured by Thermo Fisher Scientific

Phospho-mTOR is a laboratory reagent used for the detection and quantification of phosphorylated mammalian target of rapamycin (mTOR) protein. mTOR is a serine/threonine protein kinase that plays a central role in regulating cell growth, proliferation, and survival. The phospho-mTOR assay allows researchers to measure the activation status of the mTOR signaling pathway, which is implicated in various cellular processes and disease states.

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Anti-phospho-mTOR (2 citations)

2 protocols using phospho mtor

Comprehensive Immunoblotting of Cell Signaling

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Whole cell protein lysates were prepared using cell lysis buffer (Cell Signaling Technologies), 1 mM phenylmethylsulfonyl fluoride, proteasome (Roche) and a phosphatase inhibitor cocktail (Sigma-Aldrich). Proteins were resolved on 4-15% gradient gels (Biorad), transferred onto polyvinylidene fluoride membranes (Amersham), and subjected to immunoblotting using primary antibodies from Cell Signaling Technologies: p44/42 MAPK (ERK1/2; #9102), phospho-ERK1/2 (phospho-p44/42 MAPK Thr202/Tyr204; #4376), AKT (#9272), phospho-AKT (Ser473; #4060), TSC1 (#6935), phospho-TSC2 (Ser664; #40729), phospho- TSC2 (Tyr1571; #3614), TSC2 (#4308), phospho-mTOR (Ser2481; #2974), phospho-mTOR (Ser2448; #2971), mTOR (#2983), NF1 (#14623), MEK (#9122S), phosphor-MEK (#9154), vinculin (#4650); from ThermoFisher Scientific: GAPDH (#AM4300); from Millipore: anti-pan_Ras (clone RAS 10 MABS195) from Sigma: LZTR1 (HPA071248). Corresponding horseradish perodixase-conjugated secondary antibodies (Promega) were used for chemiluminescent detection.

Damnernsawad A., Bottomly D., Kurtz S.E., Eide C.A., McWeeney S.K., Tyner J.W, & Nechiporuk T. (2020). A genome-wide CRISPR screen identifies regulators of MAPK and MTOR pathways that mediate resistance to sorafenib in acute myeloid leukemia. Haematologica, 107(1), 77-85.

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Honokiol and Rapamycin Signaling Pathways

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Honokiol (HNK) and Rapamycin (RAPA) were purchased from Selleckchem. For Western blot analysis, primary antibodies against CDK2, CDK4, CDK6, Cyclin D1, p21, Bcl-2, Bcl-xL, c-Met, phospho-c-Met, HO-1, Akt, mTOR and S6 and species-specific secondary antibodies were obtained from Cell Signaling. Antibody against β-actin was purchased from Sigma Aldrich. Anti-PD-L1, phospho-Akt, phospho-S6 and phospho-mTOR antibodies were purchased from Thermo Fisher Scientific. Recombinant human hepatocyte growth factor (HGF) was purchased from Peprotech.

Sabarwal A., Chakraborty S., Mahanta S., Banerjee S., Balan M, & Pal S. (2020). A Novel Combination Treatment with Honokiol and Rapamycin Effectively Restricts c-Met-Induced Growth of Renal Cancer Cells, and also Inhibits the Expression of Tumor Cell PD-L1 Involved in Immune Escape. Cancers, 12(7), 1782.

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