Anti trim44

MiR-623 mimic and the respective controls were from GenePharma (Shanghai, China). The plasmid GV252-miR-623 expressing miR-623, lentiviral vector GV320-Ku80 and control vector were provided by GenePharma (Shanghai, China). The following siRNA target sequences were used to silence human siTRIM44 Sense: 5′-GAAUCAGUCGGAUACUCAUAG3′ and Antisense, 5′AUGAGUAUCCGACUGAUUCUG-3′. The oligonucleotides were chemically synthesized by GenePharma (Shanghai, China). Empty plasmid vector pENTER plasmid and TRIM44 overexpression plasmid pENTER-TRIM44 were purchased from Vigenebio Biosciences (Shandong, China). The oligonucleotides were chemically synthesized by GenePharma (Shanghai, China). Anti-TRIM44, anti-Vimentin, anti-MMP-2, anti-MMP-9, anti-β-actin, anti-E-cadherin and anti-N-cadherin were provided by Proteintech (Wuhan, China). Anti-Rabbit and anti-Mouse IgG were provided by Sigma (Shanghai, China).