Carbosynth

Manufactured by Merck Group

Carbosynth is a laboratory equipment product designed for carbohydrate synthesis and modification. It provides tools and reagents for the efficient synthesis of various carbohydrate-based compounds. The product's core function is to facilitate the chemical manipulation and construction of complex carbohydrate structures for research and development purposes.

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Lab products found in correlation

Annexin 5 phycoerythrin, by BD (1 mentions) Rabbit anti rad51 h 92, by Santa Cruz Biotechnology (1 mentions) Annexin 5 fluorescein isothiocyanate fitc, by BioLegend (1 mentions) Mouse anti γh2ax jbw301, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Nacalai Tesque (1 mentions) Tryptic soy broth (tsb), by Merck Group (1 mentions) Maltose, by Merck Group (1 mentions) Innova 44 incubator, by Eppendorf (1 mentions) Sucrose, by Merck Group (1 mentions)

4 protocols using carbosynth

Chemical Reagents and Standards

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All chemical products and reagents were purchased from Sigma-Aldrich (Saint-Louis, MO, USA). All chemical standards and references were purchased from Extrasynthese (Genay, France), Sigma-Aldrich or Carbosynth (Compton, United-Kingdom).

Gainche M., Ogeron C., Ripoche I., Senejoux F., Cholet J., Decombat C., Delort L., Berthon J.Y., Saunier E., Caldefie Chezet F, & Chalard P. (2021). Xanthine Oxidase Inhibitors from Filipendula ulmaria (L.) Maxim. and Their Efficient Detections by HPTLC and HPLC Analyses. Molecules, 26(7), 1939.

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Reagents and Antibodies for DNA Damage Study

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ABC, AZT, ddI, d4T, 3TC, and TDF were donated by the NIH through the AIDS reagent program. ABC was purchased from Sigma-Aldrich (SML0089) and Carbosynth (NA10019). Camptothecin (TG4110) and veliparib (ABT-888) were purchased from TopoGEN and Selleck Chemicals, respectively. These chemicals were dissolved in 100% dimethyl sulfoxide (DMSO, Nacalai Tesque) to a stock concentration of 10 to 500 mM and were stored at −20°C. Natural-type human IFN-α (Sumiferon) was purchased from Dainippon Sumitomo Pharma. Mouse anti-γH2AX (JBW301) was purchased from Merck Millipore, rabbit anti-RAD51 (H-92) was purchased from Santa Cruz Biotechnology, and rabbit anti-RAD51 (70–001) and mouse anti-RAD51 (B01P) were purchased from BioAcademia and Abnoba, respectively. Phycoerythrin–annexin V was purchased from BD Biosciences, and fluorescein isothiocyanate (FITC)–annexin V from BioLegend. PI (Nacalai Tesque) was dissolved in phosphate-buffered saline (PBS) to a stock concentration of 2 mg/ml. Rabbit anti-TDP1 (ab4166) was purchased from Abcam. Anti–β-actin (AC-15, A5441) was purchased from Sigma-Aldrich.

Tada K., Kobayashi M., Takiuchi Y., Iwai F., Sakamoto T., Nagata K., Shinohara M., Io K., Shirakawa K., Hishizawa M., Shindo K., Kadowaki N., Hirota K., Yamamoto J., Iwai S., Sasanuma H., Takeda S, & Takaori-Kondo A. (2015). Abacavir, an anti–HIV-1 drug, targets TDP1-deficient adult T cell leukemia. Science Advances, 1(3), e1400203.

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Culturing Streptomyces scabiei and Mutant

Cited 1 time

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Streptomyces scabiei 87–22 and its ΔcebR mutant [22 (link)] were routinely cultured in Tryptic Soy broth (TSB, 30 g l⁻¹, Sigma-Aldrich) or ISP2 (for 1 l: 4 g Yeast Extract, 10 g Malt Extract, 4 g Dextrose, pH 7.2) liquid media at 28 °C under shaking (180 r.p.m., New Brunswick Innova 44 incubator shaker). Modified thaxtomin defined medium (TDM) ([25 (link)], without l-Sorbose) was used as minimal medium, supplemented with maltose 0.5 % (Sigma-Aldrich) in which cellobiose (Carbosynth) and cellotriose (Megazyme) were added as inducers. Sucrose was purchased from Merck.

Deflandre B., Stulanovic N., Planckaert S., Anderssen S., Bonometti B., Karim L., Coppieters W., Devreese B, & Rigali S. (2022). The virulome of Streptomyces scabiei in response to cello-oligosaccharide elicitors. Microbial Genomics, 8(1), 000760.

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Enzymatic Characterization of BglX

Cited 1 time

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We analyzed the ability of BglX to process a variety of carbohydrate molecules (structures are shown in Figure 2A and Figure S1). Eleven peptidoglycans were synthesized in our laboratories.^{8 ,35 (link)-37 (link)} The remaining saccharides are commercial (Sigma-Aldrich, Carbosynth). Reaction of each compound with recombinant wild-type BglX was carried out in 20 mM HEPES, 0.1 M NaCl, pH 7.0 buffer at 37 °C. After 18 h, the reaction mixtures were flash-frozen. The reaction mixtures were analyzed by LC/MS (Figure 2 and Figures S2 and S3). The LC/MS instrument conditions are listed in Table S2.

Mahasenan K.V., Batuecas M.T., De Benedetti S., Kim C., Rana N., Lee M., Hesek D., Fisher J.F., Sanz-Aparicio J., Hermoso J.A, & Mobashery S. (2020). Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implications for Biofilm Formation. ACS chemical biology, 15(1), 189-196.

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